摘要
目的以市售血栓通胶囊为参比制剂,研究三七总皂苷类脂质体(PNSN)抗急性心肌缺血药效。方法将50只大鼠随机分为5组,每组10只,分别为空白对照组、模型组、血栓通胶囊组(54 mg·kg^(-1))、PNSN低剂量组(54 mg·kg^(-1))、PNSN高剂量组(100 mg·kg^(-1))。血栓通胶囊组和PNS类脂质体高、低剂量组大鼠均灌胃给予相应药物,每天1次,连续7 d;模型组、空白对照组大鼠灌胃等体积蒸馏水(2mL·kg^(-1))。第5 d除空白对照组外,其余各组在给药后1 h分别腹腔注射异丙肾上腺素10 mg·kg^(-1)、5 mg·kg^(-1)、5 mg·kg^(-1),每天1次,连续3 d。末次腹腔注射异丙肾上腺素20 min后,用10%水合氯醛麻醉,腹主动脉取血后试剂盒ELISA测定。结果与血栓通组比较,PNSN低、高剂量组丙二醛(MDA)含量极显著性降低,PNSN高剂量组游离脂肪酸(FFA)含量显著性降低。结论 PNSN具有缓释和保护受损心肌细胞的作用。
OBJECTIVE To study the effect of Panax Notoginsenosides Liposome （PNSN） on the effect of acute myocardial ischemia by using the commercially available thrombus capsule as the reference preparation. METHODS 50 rats were randomly divided into blank control group, model group, thrombosis capsule group （54 mg·kg^-1） ,PNSN low dose group （54 mg·kg^-1） ,PNSN high dose group （100 mg·kg^-1）. Rats of thrombosis capsule group,PNSN low dose group ,PNSN high dose group were administered with corresponding drugs, 1 times a day, continuous 7 d. Rats in model group and blank control group were filled with distilled water （2 mL·kg^-1）.Fifth days in addition to the blank control group, the other groups were injected with 1 h in the intraperitoneal injection of ISO 10 mg·kg^-1、5 mg ·kg^-1、5 mg·kg^-1 1 times a day, continuous 3 d. After the last intraperitoneal injection of ISO 20 min, anesthesia with 10% hydrate, ELISA determine for abdominal aorta. RESULTS Compared with thrombosis group, PNSN low and high dose group significantly decreased the content of malondialdehyde （MDA）, and the content of free fatty acid （FFA） in PNSN high dose group was significantly decreased. CONCLUSION PNSN has slow release effect and has the function of protecting the injured cardiomyocytes.
出处
《人参研究》
2017年第1期21-24,共4页
Ginseng Research
基金
吉林省科技发展计划项目
项目编号:20130727093YY
关键词
三七总皂苷
类脂质体
药效验证
Panax Notoginsenosides
liposomes
comparison of drug effects
