摘要
目的改变红细胞电荷性质,克服因红细胞与单链DNA均带负电荷而引起的排斥力,达到单链DNA易与红细胞靠近并特异结合的目的。方法以不改变红细胞电荷性质的生理盐水及低离子强度液反应介质为对照组,使用凝聚胺溶液反应介质为试验组,在室温下与ss DNA孵育30 min,并定量检测各组结合于红细胞的单链DNA拷贝数。结果在生理盐水、LISS液、凝聚胺溶液反应介质中,与红细胞结合的单链DNA拷贝数分别为(4.22±2.17)×10~7/μL、(6.13±5.50)×10~7/μL、(8.54±2.97)×10^(10)/μL(n=9)。试验组与对照组间比较P<0.05。结论在凝聚胺溶液反应介质中,携带负电荷的ssDNA可更好地与红细胞结合。与对照组相比,试验组中结合于红细胞的单链DNA拷贝数量级数可提高10~3拷贝/μL。
Objective To change the charge property of red blood cells to overcome the electrostatic repulsive force between the red blood cells and ss DNA. Methods The normal saline and low ionic strength solution reaction medium which did not affect the nature of the red blood cell charge acted as control group,and polybrene solution reaction medium was designed as the experiment group. After the red blood cells were incubated with ss DNA at room temperature for 30 minutes,ssDNA copies agglutinated with red blood cells were detected. Results The ss DNA copies agglutinated with the red blood cells in normal saline,the low ionic strength solution and polybrene solution reaction medium,which were（ 4. 22 ± 2. 17） × 10^7/μL,（ 6. 13±5. 50） ×10^7/μL,（ 8. 54±2. 97） ×10^（10）/μL（ n = 9）,respectively. There was statistically significant difference between the experiment group and control group（ P 0. 001）. Conclusion The polybrene solution reaction medium was helpful to the agglutination between ss DNA and red blood cells,The ss DNA copies could improve by 103/μL in a polybrene solution medium compared with the control group.
出处
《中国输血杂志》
CAS
北大核心
2017年第2期180-182,共3页
Chinese Journal of Blood Transfusion
