摘要
背景:前期研究发现,骨骼肌卫星胞移植能够诱导心肌梗死区新生血管形成,缩小梗死面积,改善其心功能,但整体效果并不太理想。目的:观察碱性成纤维细胞生长因子基因修饰骨骼肌卫星细胞在急性心肌梗死区的存活及对心肌梗死区血管新生的影响。方法:将18只新西兰大白兔随机分为3组,实验组、对照组结扎冠状动脉左前降支,构建急性心肌梗死动物模型;空白对照组只穿线,不结扎。造模成功即刻,实验组于局部梗死心肌内注射DAPI标记的碱性成纤维细胞生长因子基因修饰自体骨骼肌卫星细胞悬液50μL,对照组注射等量DAPI标记的自体骨骼肌卫星细胞。细胞移植4周后取标本,观察心肌梗死区骨骼肌卫星细胞存活及成纤维细胞生长因子基因表达情况,免疫组织化学染色检查心肌梗死区新生血管形成情况。结果与结论:(1)空白对照组未见DAPI标记的细胞,对照组及实验组缺血心肌区域均可见大量DAPI标记的骨骼肌卫星细胞,实验组还可见大量EGFP-碱性成纤维细胞生长因子融合蛋白绿色荧光表达;(2)实验组、对照组新生微血管密度多于空白对照组(P<0.05),实验组新生微血管密度多于对照组(P<0.05);(3)结果表明,碱性成纤维细胞生长因子修饰骨骼肌卫星细胞可在急性心肌梗死区存活,促进心肌梗死区血管新生。
BACKGROUND： Previous studies have found that skeletal muscle satellite cell transplantation can induce angiogenesis in myocardial infarction area, reduce infarct size and improve cardiac function. But the overall effect is not satisfactory. OBJECTIVE： To observe the survival of basic fibroblast growth factor（bFGF） gene modified skeletal muscle satellite cells in acute myocardial infarction and to observe the expression of b FGF gene and the effect of cell transplantation on angiogenesis in myocardial infarction area.METHODS：Eighteen New Zealand white rabbits were divided into three groups by random：skeletal muscle satellite cell group（control group）,b FGF gene enhanced skeletal muscle satellite cell group（experimental group）and blank control group.The left anterior descending branch of the coronary artery of the rabbits was ligated so as to establish an animal model of acute myocardial infarction in the former two groups.After labeled by DAPI before transplantation,the skeletal muscle satellite cells,b FGF gene modified skeletal muscle satellite cells and the equivalent amount of DMEM/F12 were injected into the local infarct myocardium correspondingly.Samples were taken 4 weeks after transplantation.Then,the survival of skeletal muscle satellite cells and the expression of b FGF gene were observed under light microscope and fluorescence microscope,and the neovascularization in the myocardial infarction area was examined by immunohistochemical staining.RESULTS AND CONCLUSION：No DAPI-labeled cells were visible in the blank control group,but in the other two groups,a large amount of DAPI-labeled skeletal muscle satellite cells were seen in the infarction area.Enhanced green fluorescent protein was highly expressed in the experimental group.Microvessel density in the infarction area was highest in the experimental group followed by the control and blank control groups（P〈0.05）.These findings indicate that b FGF gene modified skeletal muscle satellite cells can survive and promote neovascula
出处
《中国组织工程研究》
CAS
北大核心
2017年第9期1397-1401,共5页
Chinese Journal of Tissue Engineering Research
基金
深圳市科技计划项目(200404104)~~
