摘要
目的观察不同浓度阿霉素对小鼠T淋巴细胞白血病YAC-1细胞增殖及凋亡的影响,探讨给药后阿霉素在白血病细胞中相对含量变化。方法培养YAC-1细胞,加入分别加入不同浓度(0.3125、0.625、1.25、2.5 h和5μmol/L)的阿霉素,继续培养24 h,显微镜下观察细胞形态,采用流式细胞术检测细胞周期、细胞凋亡和阿霉素红色荧光,红色荧光强度可指示细胞内药物含量。不同浓度阿霉素处理细胞72 h,台盼蓝染色法计数细胞并计算细胞增殖抑制率。结果高浓度阿霉素(2.5和5μmol/L)导致细胞肿胀、胞浆内空泡增加;阿霉素以浓度依赖的方式抑制YAC-1细胞增殖;凋亡及坏死百分率以及细胞内阿霉素含量呈现剂量依赖性增加。结论 YAC-1细胞对阿霉素敏感,采用流式细胞术可实现对细胞内药物含量和抗肿瘤效应的定量分析。
Objective To analyze the cell proliferation inhibition and amycin （ADR） on mouse T lymphoblastic leukemia cells YAC-1. And apoptosis effects of different concentration adri- the intracellular relative amount of ADR in leu- kemia cells will be also measured. Methods The YAC-1 cells were cultured and treated by different concentration of ADR （0.3125,0. 625,1.25,2.5 h and 5 μmol/L）for 24 hours, and the cells morphology were observed under the mi- croscope. The cell cycle,cell apoptosis and the red fluorescence of ADR in cells were measured by aClc^w cytometry. The intensity of red fluorescence indicated the relative amount of ADR. Cells were stained by trypan blue and counted after treated by ADR for 72 hours, thus cell proliferation rates were also calculated. Results Cellular swelling and more vacuoles intracellor were observed after treated by higher concentration of ADR which inhibited YAC-1 cells prolifera- tion in concentration dependent manner. The percentage of apoptosis or necrosis cells and intracellular relative amount were also increased with ADR concentration increased. Conclusion YAC-1 cells are sensitive to ADR, while both the intracellular relative amount of ADR and the tumor therapy effects could be determined quantitatively.
作者
史立言
陈为
马畅
曹秀琪
刘岩
李妍
SHI Liyan CHEN Wei MA Chang CAO Xiuqi Liu Yan LI Yan(The Medical College of Yan Bian Uni versity, Yan Ji, Jilin Province, 133002 The Medical Laborotory College of Jilin Medical University, Jilin City, Jilin Province, 132013, China)
出处
《吉林医药学院学报》
2017年第2期84-86,共3页
Journal of Jilin Medical University
基金
吉林省科技厅重大项目资助课题(20140203012YY)
吉林省卫生厅项目资助课题(2012Z06)
吉林省大学生创新创业项目
关键词
阿霉素
细胞周期
凋亡
adriamycin
cell cycle
apoptosis
flow cytometry
