摘要
目的:观察压力性尿失禁大鼠尿道组织神经肽Y(NPY)及蛋白基因产物9.5(PGP9.5)表达的变化以及腰骶部推拿和腹部推拿对其产生的影响,为压力性尿失禁的推拿手法治疗提供一定的研究依据。方法:取SUI模型大鼠、正常大鼠、腹部推拿治疗大鼠、腰骶部推拿治疗大鼠的尿道标本并用免疫组化的方法检测标本中NPY、PGP9.5的表达情况。结果:通过模拟产伤和绝经可以获得压力性尿失禁的动物模型。免疫组化染色显示,NPY和PGP9.5存在于尿道组织中,且模型对照组与空白对照组、腹部推拿组以及腰骶部推拿组相比较,模型组中NPY和PGP9.5染色强度显著降低(P<0.05)。腹部推拿组与腰骶部推拿组NPY的阳性表达差异无统计学意义。腹部推拿组比腰骶部推拿组PGP9.5的阳性表达显著降低(P<0.05)。结论:腰腹部推拿对SUI的治疗作用可能与其对神经损伤的修复有关。体现了"从阴引阳,从阳引阴"理论。
Objective： To observe changes of the expression of neuropeptide Y（NPY） and protein gene product 9. 5（PGP9. 5） in urethral tissue of rats with stress urinary incontinence（SUI） and the effect of tuina on abdomen or lumbago,providing research evidence for tuina therapy in treating SUI. Methods： Samples at urethra were obtained from the rat models of SUI,normal rats,tuina on abdomen rats and tuina on lumbago rats and the expression levels of NPY and PGP9. 5 were detected by immunohistochemistry.Results： The rat models of SUI were established create birth injury and menopause. Immunohistochemistry showed that NPY and PGP9. 5 existed in urethral tissue,and the staining intensities of NPY and PGP9. 5 in rat model group decreased significantly compared with normal control group,tuina on abdomen group and tuina on lumbago group（P〈 0. 05）. There was no significant difference of the expression of NPY between tuina on abdomen group and tuina on lumbago group. Compared with tuina on lumbago group,the expression of PGP9. 5 in tuina on abdomen group decreased significantly（P〈 0. 05）. Conclusion： The therapeutic effects of tuina on abdomen or lumbago for SUI may have certain connection with repairing the neurogenic injury. This therapy reflects the theory of ＂inducing Yang from Yin and inducing Yin from Yang＂.
出处
《世界中医药》
CAS
2016年第10期2101-2104,2108,共5页
World Chinese Medicine
基金
北京市自然科学基金项目(编号:7143187)
