摘要
本文采用石油醚(30~60℃)、乙酸乙酯、二氯甲烷、正丁醇和乙醇5种溶剂依次对暗紫贝母内生真菌Fusarium sp.A14发酵液进行提取,获得5种不同极性馏分。用DPPH·、ABTS^+·清除活性和FRAP活性对其进行了抗氧化活性评价,用福林酚法和NaNO_2-Al(NO_3)_3-Na OH比色法测定了各馏分中总多酚含量(TPC)和总黄酮含量(TFC),最后用TLC自显影法和HPLC法分析抗氧化活性成分。抗氧化活性及TPC和TFC测定结果表明,后四种溶剂提取馏分具良好的抗氧化活性,以乙酸乙酯和正丁醇提取馏分活性最强,且抗氧化活性与TPC和TFC存在明显相关性。此外,TLC自显影分析结果表明抗氧化活性成分具有多样性;HPLC分析结果表明乙酸乙酯和二氯甲烷中馏分中有多种具较强抗氧化活性的酚酸类物质,如儿茶素、咖啡酸、木樨草素和阿魏酸。本研究表明:暗紫贝母内生真菌Fusarium sp.A14具有能够生产已知或者未知抗氧化活性物质的潜力,其在医药健康领域具有重要应用价值。本试验为其进一步开发和利用奠定了基础。
To study the antioxidant activity and the active ingredients of endophytic fungal Fusarium sp. A14 isolated form Fritillaria unibracteata Hsiao et KC Hsia（FUB） , the petroleum ether（ 30-60 ℃ ） , ethyl acetate, dichloromethane, n- butyl alcohol and ethanol were used to extract the metabolic products of Fusarium sp. A14. Then, the antioxidant activi- ties of the five fractions were evaluated using DPPH scavenging assay,ABTS＋ scavenging assay and ferric reducing- antioxidant power（FRAP） assay. The total polyphenols content（TPC） and total flavonoids content（TFC） of the five fractions were evaluated using folin-reagent method and NaNO-A1 （NO3）3-NaOH colorimetrie method, respectively. The antioxidant constituents were analyzed further by TLC-bioautography and high-performance liquid chromatography （HPLC） methods. The results of antioxidant assay, TPC and TFC indicated that the last four fractions had strong antioxi- dant activities, which possessed positive correlations with the TPC and TFC to some degrees. The TLC-bioautography re- sults exhibited that there were abundant diverse chemical components with antioxidant activity. In addition, HPLC analy- sis result illustrated that there were multiple phenolic acids in the ethyl acetate and dichloromethane fractions, such as catechin, caffeic acid, luteolin and ferulic acid. Therefore, the present study demonstrated that the fungal endophytic Frit- illaria sp. A14 from FUB had the potential production of partial known and unknown antioxidants and which could be ap- plied in the health and medical profession. This study will contribute to the development and utilization of natural product from Fritillaria sp. A14.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2017年第3期376-381,392,共7页
Natural Product Research and Development
基金
高等学校博士学科点专项科研基金(20115103110009
关键词
暗紫贝母
内生真菌抗氧化
总多酚
总黄酮
TLC自显影
HPLC
Fritillaria unibracteata Hsiao et KC Hsia
antioxidant activity of endophytic fungus
total phenolics content
total flavonoids content
TLC-bioautography
HPLC
