摘要
条锈病是由小麦条锈菌（Puccinia striiformis f.sp.tritici）引起的世界范围内小麦上最重要的病害之一。利用抗病品种是防治该病最经济、有效的措施。但是由于小麦条锈菌的高度变异性,品种抗病性很容易被条锈菌新小种所克服。因此,持续监测条锈菌生理小种的动态变化,及时发现新小种,
Stripe rust,caused by Puccinia striiformis f. sp. tritici（Pst）,is one of the most important diseases on wheat in China. Zhong 4,a partial amphiploid（2n=56） carrying resistance from Thinopyrum intermedium and resistant to most of Pst pathotypes identified so far. T4 is one of the very rare Pst pathotypes which are virulent to Zhong 4. With the aim of developing race-specific molecular markers for detection of Pst pathotype T4,214 random amplified polymorphic DNA（RAPD） primers were used to test polymorphism among pathotype T4 and six predominant pathotypes CYR29,CYR31,CYR32,CYR33,Su11-4 and V26. An unique 821 bp band was identified in T4 with primer S1363. The band was cloned and sequenced. Based on the sequences,sequence characterized amplified region（SCAR） marker T4sp1/sp2 was developed to differentiate T4. The SCAR marker was then validated with DNA samples prepared from leaves of wheat cultivar Mingxian 169 after inoculated with T4,CYR29,CYR31,CYR32,CYR33,Su11-4,V26 and water（mock-inoculated）. The specific band was produced only in samples prepared from plants after inoculated with T4 six days. Therefore,the SCAR marker T4sp1/sp2 can specifically detect T4.
出处
《植物病理学报》
CAS
CSCD
北大核心
2017年第2期270-273,共4页
Acta Phytopathologica Sinica
基金
国家"973"计划(2013CB127700)
国家"十二五"科技支撑计划(2012BAD19B04)
国家自然科学基金(31071652)
高等学校学科创新引智计划(B07049)
陕西省自然科学基金(2015JM3075)
关键词
小麦条锈菌
SCAR
开发
标记
检测
菌系
T4
感染
Puccinia striiformis f.sp.tritici
T4 pathotype
SCAR marker
molecular detection
