摘要
目的探索汉族群体中甲基苯丙胺依赖相关的差异表达基因。方法采集14例汉族甲基苯丙胺依赖者以及8例年龄、性别相匹配正常人的外周全血,利用mRNA表达谱芯片对其进行基因表达谱分析,并对差异表达基因进行功能分类分析。结果在27 958条转录本中,实验组和对照组差异表达倍数在2倍或2倍以上的转录本有3 560条,其中上调表达基因1 510个,下调表达基因2 576个。对这些差异基因分析发现甲基苯丙胺对细胞组分、分子功能、生物学过程的负调节有重要影响,并与转录调控、核苷酸代谢和修饰、蛋白质修饰、细胞凋亡、应激调节、自身免疫反应等相关。结论甲基苯丙胺成瘾的发生涉及多基因表达的改变,芯片技术可以有效地筛选出甲基苯丙胺依赖者与正常对照的差异表达基因,对进一步探索甲基苯丙胺成瘾机制、有效的干预或逆转毒品成瘾具有重要意义。
Objective To explore the differentially expressed genes related to methamphetamine dependence among the Han nationality. Methods Whole Human Genome Oligo Microarray was used to screen for differentially expressed genes in peripheral blood cells between 14 cases selected from Methamphetamine addiction group and 8 cases of healthy controls. Differentially expressed genes were fuctionally discrimated. Results Among the 27,958 Entrez Gene RNAs,there were 3560 differentially expressed genes which different expression have two time or above difference between treatment group and control group,and among which 1510 were up-regulated and 2576 were down-regulated genes. Gene Ontology enrichment of genes that significantly differentially expressed between Methamphetamine addiction people and healthy controls in Han people showed that Methamphetamine played an important role in the negative regulation of molecular function,biological processes,and cellular component,which were related to transcriptional control,protein modification,apoptosis,regulation of cellular response to stress,immunoreaction. Conclusion Differentially expressed genes between Methamphetamine addiction people and healthy people can be effectively identified using genome oligonucleotide microarray analysis. The study supplies a list of potential gene candidates which may play important roles on Methamphetamine-mediated-pathogenesis development and/or may be used as the target for infection treatment in the future studies.
出处
《毒理学杂志》
CAS
CSCD
北大核心
2017年第1期25-29,共5页
Journal of Toxicology
基金
南京森林警察学院国家自然科学基金预研项目(LGY201502)
