摘要
为研究水杨酸(Salicylic acid,SA)处理对蓝莓(Vaccinium spp.)耐热性的影响,将经0.1 mmol/L SA预处理的盆栽蓝莓V3和Misty品种植株置于人工气候箱内进行热胁迫处理,测定蓝莓叶片的相关生理指标.结果表明:42℃热胁迫可使蓝莓植株叶片发黄和萎蔫,而SA处理则可有效缓解此现象;与热胁迫空白对照相比,SA处理可降低热胁迫蓝莓脯氨酸的质量分数,并在热胁迫96 h时降幅最大,V3、Misty品种的降幅分别是10.47%和30.11%;SA处理可降低蓝莓的相对电导率,在热胁迫96 h,V3品种的相对电导率降幅达到22.52%,Misty品种达到20.5%;SA处理可减缓蓝莓叶片的丙二醛(Malonaldehyde,MDA)积累,其中Misty品种的MDA降幅大于V3品种;SA处理可明显增加蓝莓叶片的超氧化物歧化酶(Superoxide dismutase,SOD)活性,热胁迫96 h时Misty品种的SOD活性的升幅大于V3品种;在热胁迫后期(96 h),SA处理可降低蓝莓植株的叶绿素质量分数降幅,且V3品种叶绿素质量分数降幅小于Misty品种.由此认为,SA处理可改善蓝V3和Misty品种的耐热性,其中V3品种的耐热性优于Misty品种.
In order to study the effects of salicylic acid （SA） treatment on heat tolerance of blueberry （ Vaccinium spp. ） , two cultivars V3 and Misty of blueberry treated by 0. 1 mmol/L SA were subjected to heat stress trentment, and their physiological indexes were measured. Heat stress with 42 ℃ led to blueberry leaf yellowing and wilting, in manual climatic chamber. However, 0. 1 mmol/L SA treatment could alleviate effectively the above symptom. Proline contents declined with SA treatment compared with heat stress control （control 2）. The biggest drop occurred at 96 h, decrease of V3 and Misty were 10.47% and 30. 11%, respectively; relative electroconduetibilities declined with SA treatment compared with control 2. The biggest drop appeared at 96 h. Decrease of V3 was 22.52% , and the decrease of Misty were 18.5% at 24 h, 20.5% at 96 h. SA alleviated the damage of cell membrane integrity via decreasing the contents of Malonic dialdehyde （MDA）. The MDA of Misty decreased more significantly than that of V3 during heat stress. SA was able to stimulate the antioxidant system via increasing Superoxide dismutase （SOD） activity for improving the heat resistances of blueberry. Compared with the control, SOD activity of the two cultivars at 96 h increased more significantly than that at 24 h during heat stress. Thus, SA was able to stimulate SOD activity and regulate the osmotic balance for improving the heat resistances of blueberry, as a result of alleviating the damage of the cell membrane, decreasing the chlorophyll degradation and protecting the physiological function of the normal protein. Misty was more sensitive than V3 to heat stress and SA treatment.
作者
刘凤民
贾慧颖
文了
黎海燕
罗文亮
张伟丽
LIU Fengmin JIA Huiying WEN Liao LI Haiyan LUO Wenliang ZHANG Weili(Teaching and Research Management Center, Zhongkai University of Agrieuhure and Engineering, Guangzhou 510225, China College of Life Sciences, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China)
出处
《仲恺农业工程学院学报》
CAS
2017年第1期8-12,共5页
Journal of Zhongkai University of Agriculture and Engineering
基金
广东省科技计划(2013B020304007)
仲恺农业工程学院2015大学生创新基金(2015A09)资助项目
