摘要
为探讨下丘脑-垂体-甲状腺轴对斗鸡攻击行为的影响,采用免疫组织化学法对比分析不同生长阶段吐鲁番斗鸡与新罗曼蛋鸡下丘脑中促甲状腺激素释放激素(TRH)神经元的数量,结合酶联免疫吸附法测定两品种成年公鸡攻击行为表现前、中、后各时期血清中TRH和甲状腺激素(TH)的含量。结果表明两品种鸡的TRH在下丘脑中的阳性细胞数随着周龄增加而减少,整体上吐鲁番斗鸡TRH的阳性细胞数高于新罗曼蛋鸡,公鸡高于母鸡;斗鸡和新罗曼蛋鸡血清中TRH的含量随着周龄增加逐渐上升,TH呈下降趋势,且吐鲁番斗公鸡的TRH和TH含量较高,打斗时间的持续性也比新罗曼蛋公鸡长。因此,吐鲁番斗鸡较强的攻击行为可能与这两种激素含量偏高有关。
The present study aims to investigate the effect of hypothalamus-pituitary-thyroid axis on gamecock aggressive behavior.The hypothalamus of Turpan gamecocks and New Roman layers at different growth stages were extracted,respectively.The numbers of thyrotropinreleasing hormone（TRH） positive neurons were detected by immunohistochemistry method.ELISA was used to measure the concentrations of TRH and thyroid hormone（TH） in serum at different weeks during cockfight and layer rooster attacking before,during and after each period.The results showed that the number of TRH-positive cells in the hypothalamus was decreased with ages,and Turpan gamecock exhibited slightly higher number of TRH-positive cells than New Roman layers.In the two species of chickens,serum concentrations of TRH increased gradually while TH level decreased.Serum TRH and TH concentrations were higher in Turpan rooster than that of gamecock rooster.However,the fighting duration of gamecock rooster is longer than that of New Roman rooster.Therefore,it is reasonable to speculate that the strong aggressive behavior of Turpan gamecock may be related to the high levels of TRH and TH in the circulating system.
出处
《畜牧与兽医》
北大核心
2017年第3期52-56,共5页
Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金项目(31360545)
石河子大学高层次人才项目(RCZX201101)
