摘要
目的:研究长非编码RNA BANCR对人非小细胞肺癌耐顺铂细胞株A549/DDP耐药性的影响及其可能的作用机制。方法:应用实时荧光定量PCR法(q RT-PCR)检测耐顺铂细胞株A549/DDP及其亲本细胞株A549中BANCR的表达差异,发现BANCR在A549/DDP细胞中显著低表达。在A549/DDP细胞中过表达BANCR,分别应用MTT法、克隆形成实验、流式细胞术检测过表达后A549/DDP细胞对顺铂药物敏感性,细胞增殖能力,联合顺铂处理后细胞凋亡变化;Western blot检测过表达BANCR后A549/DDP细胞p53的表达变化。结果:BANCR在A549/DDP细胞中的表达量显著低于A549细胞(P<0.05),在A549/DDP细胞中过表达BANCR可产生以下效应:相较于对照组,顺铂对A549/DDP/BANCR细胞的半数抑制浓度(half inhibition concentration,IC50)减低(P<0.05),A549/DDP/BANCR细胞的增殖能力减弱,A549/DDP/BANCR经过顺铂处理后细胞凋亡增多(P<0.05)。Western blot结果显示,与空白对照组比较,过表达BANCR的A549/DDP细胞p53表达水平明显升高。结论:BANCR可能通过诱导细胞凋亡,抑制细胞增殖,上调p53蛋白表达而逆转A549/DDP细胞对DDP的耐药性。
Objective：To explore the effect of BANCR in reversing cisplatin（DDP） resistance of human non-small cell lung cancer（NSCLC）A549/DDP cell line and its related mechanisms. Methods：The real-time fluorescent quantitative PCR（q RT-PCR） was used to detect BANCR expression differences in DDP-resistant A549/DDP cells and its parent cell line A549.Overexpress BANCR in A549/DDP cells and use MTT assay,colony formation assay and flow-cytometric analysis to detect A549/DDP cells to DDP drug sensitivity,cell proliferation ability and apoptosis on treatment with DDP.The protein expression of p53 in A549/DDP cells were detected by western blotting. Results：The BANCR expression level in A549/DDP cell line was significantly lower than that in A549 cell line（P〈0.05）. Ectopic BANCR expression in A549/DDP cells could produce the following effects：compared to the control group,the half inhibition concentration（IC50） of DDP in A549/DDP/BANCR cells lower（P〈0.05）,proliferation of A549/DDP/BANCR cells diminished（P〈0.05）,apoptosis on treatment with DDP increased（P〈0.05）. Furthermore,the protein expressions p53 was upregulated in BANCR transfected group than those in empty vector. Conclusion：BANCR reverses the resistance of A549/DDP cells to DDP through induction of apoptosis,diminished cell proliferation,up-regulating the expressions of p53 protein.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2017年第2期189-193,共5页
Journal of Nanjing Medical University(Natural Sciences)
基金
国家自然科学基金(81272601
81472198)
江苏省科技厅临床医学科技专项(BL2014096)
江苏省医学重点人才基金(RC2011080)
