摘要
随着基因序列分析研究的深入,中短基因片段的快速准确测序已经成为生物信息研究的瓶颈。本文针对第二代基因测序仪的硬件设计问题,在深入研究第二代基因测序原理和流程的基础上完成了硬件总体方案设计。此方案采用自上而下逐步分解的方法,设计了流动槽、PCR、边合成边测序、控制与数据传输等硬件模块,继续分解设计了温度控制、试剂控制、激光触发、光学采集、扫描控制等硬件子模块,并根据各个子模块的功能和厂商的产品说明书完成了部分关键器件的选型。为后续基因测序仪的机械结构设计和硬件组装奠定了基础。
With the development of genomics,fast and accurate short DNA fragments sequencing has become the bottleneck of bioinformatics research.In this paper,theory and method of high throughput sequencing is deeply studied.Based on top-down policy,four modules and eight sub-modules have been designed,which are flow cell,PCR,sequencing-by-synthesis and control modules with temperature control,reagent control,laser control,optical collection,scanning control,device control,image processing and data transmission sub-modules.In the end,the main hardware parts have also been selected according to the functions of submodules and product specification.This work will be the basis of further mechanism design and hardware assembling of sequencing instrument.
出处
《生命科学仪器》
2017年第1期43-45,42,共4页
Life Science Instruments
基金
高等学校博士学科点专项科研基金(20110201110010)
关键词
生物信息学
基因测序仪
边合成边测序
硬件设计
bioinformatics
DNA sequencing instrument
Sequencing-by-Synthesis
Hardware design
