摘要
目的建立兔耳创面修复瘢痕模型,探讨在瘢痕形成过程中肿瘤坏死因子α刺激基因6(TSG-6)对基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶抑制剂-2(TIMP-2)表达和瘢痕修复的影响。方法参照Morris方法,建立兔耳创面模型,设计实验组,注射TSG-6;对照组注射等量的无菌生理盐水。检测各组瘢痕指数及Ⅰ、Ⅲ型胶原的表达情况来评价瘢痕增生情况。同时检测MMP-2及TIMP-2基因的表达情况。结果实验组的瘢痕指数及Ⅰ、Ⅲ型胶原含量低于对照组,MMP-2的表达较多,对应的TIMP-2表达降低,差异有统计学意义(P<0.05)。结论 TSG-6可以抑制瘢痕增生,可能是增多瘢痕组织中MMP-2表达来实现的。
Objective To investigate the influence of TSG-6 on MMP-2 and TIMP-2 expression and scar repairing in cicatrization in the model of rabbit ear scar.Methods A rabbit ear scar model was established according to the Morris method.The left ear was selected as the experimental group and injected with TSG-6,and the right ear,as the control group,was injected with the same dose of sterile normal saline.The condition of scar hyperplasia was evaluated by scar index and Ⅰ and Ⅲ collagen expression.The expression of MMP-2 and TIMP-2 gene was also tested.Results The scar index and Ⅰ and Ⅲ collagen content in the experimental group were less than that in the control group.MMP-2 expressed relatively higher and TIMP-2 expressed lower.The difference was statistically significant （P〈0.05）.Conclusion Scar hyperplasia can be inhibited by TSG-6.The mechanism increased MMP-2 expression in cicatricial tissue.
出处
《中国美容整形外科杂志》
CAS
2017年第3期170-172,共3页
Chinese Journal of Aesthetic and Plastic Surgery
基金
四川省教育厅科研项目(16ZB0287)
