摘要
目的探讨侧脑室注射N-甲基-D-天冬氨酸受体2B(NR2B)拮抗剂Ro25-6981对大鼠脑局灶性缺血-再灌注(I-R)损伤的影响。方法60只雄性SD大鼠随机均分为四组:假手术组(S组),I-R组,I-R+Ro25-6981组(I-R+Ro组)和I-R+生理盐水组(I-R+NS组)。S组大鼠线栓置入颈内动脉的深度为10mm;I-R组大鼠采用线栓法阻闭大脑中动脉,2h后拔出线栓到颈外动脉给予再灌注;I-R+Ro组和I-R+NS组于缺血后2h脑室内分别注射Ro25-6981 0.1mol/L和生理盐水各5μl。于再灌注24h后行神经功能缺损评分;麻醉大鼠取脑组织,切片后用2,3,5-氯化三苯基四氮唑染色,Image Pro Plus 6.0图像处理软件计算大鼠脑梗死容积。结果与S组相比,I-R组大鼠神经功能缺陷评分增加,脑梗死容积增大(P<0.05或P<0.01);与I-R组相比,I-R+Ro组大鼠神经功能缺损评分降低,脑梗死容积减小(P<0.05或P<0.01)。结论大鼠缺血2h后脑室内注射NR2B拮抗剂Ro25-6981可以降低神经功能缺损评分、缩小脑梗死容积,对大鼠局灶性脑I-R损伤有一定保护作用。
Objective To explore the neuroprotective effects of N-methyl-D-aspartate receptor2B（NR2B）antagonist Ro25-6981 on focal cerebral ischemia-reperfusion（I-R）injury in rats.Methods Sixty SD rats were randomly and equally divided into 4groups of S（sham operated）,I-R,I-R＋Ro and I-R＋NS.The middle cerebral artery in rats was occluded for 120 minutes by an intraluminal filament and then reperfused to cause transient focal cerebral ischemia in groups of I-R,I-R＋Ro and I-R＋NS.Ro25-6981 and normal saline were injected intracerebroventricularly at 120 minutes after ischemia in groups of I-R＋Ro and I-R＋NS,respectively.The neurological deficit score of rats was evaluated at24 hours after I-R injury.Then the rats were deeply anesthetized and the brains were removed for making pathological sections stained by 2,3,5-triphenyl tetrazolium chloride.The infarct volume was calculated by Image Pro Plus soft 6.0.Results Compared with group S,the infarct volume and neurological deficit score were increased in group I-R（P〈0.05 or P〈0.01）.Compared with group I-R,the infarct volume and neurological deficit score were decreased in group I-R＋Ro（P〈0.05 or P〈0.01）.Conclusion Intracerebroventricular administration of NR2 Bantagonist Ro25-6981 at 120minutes after ischemia possesses neuroprotective effects on focal cerebral I-R injury,which can reduce the infarct volume and neurological deficit score.
出处
《江苏医药》
CAS
2017年第5期305-307,F0002,共4页
Jiangsu Medical Journal
基金
国家自然科学基金(81471835
81471136)
