摘要
目的:研究牙龈卟啉单胞菌脂多糖(Pg-LPS)对小鼠骨髓基质细胞系ST-2在成骨分化过程中Eph受体酪氨酸激酶A4基因(EphA4)表达的影响。方法:使用终浓度10μg/m L的Pg-LPS与ST-2细胞共培养,分别在培养第1、3和7天,采用RT-PCR技术检测EphA4基因及Runt相关转录因子2(Runx2)、Ⅰ型胶原蛋白(ColⅠ)和碱性磷酸酶(ALP)等成骨相关基因的表达。结果:实验组EphA4基因表达在培养第1、3和7天时比对照组分别减少2.5、2.4和2.3倍,两组之间存在显著差异(P<0.01);实验组Runx2基因表达在培养第1、3天比对照组减少,两组之间存在显著差异(P<0.01),但在第7天两组表达均不明显;实验组ColⅠ和ALP基因表达在培养第1、3和7天时均比对照组减少,差异有统计学意义(P<0.01)。结论:Pg-LPS能抑制ST-2细胞成骨分化过程中EphA4基因的表达,同时也抑制成骨相关基因Runx2、ColⅠ和ALP的表达。
Objective: To observe the effect of Porphyromonas gingivalis lipopolysaccharide ( Pg-LPS) on the expression of EphA4 in mouse marrow stromal cell line ST-2. Methods: ST-2 cells were co-cultured with 10 μg/mL of Pg-LPS at 1 d,3d and 7 d. EphA4 gene and the osteogenic related genes ( Runx2, Col /and ALP) were detected by RT-PCR. Results: Compared with Pg-LPS group, at the 1 d,3 d and 7 d,the EphA4 mRNA expression was significantly decreased by 2.5 folds, 2.4 folds and 2.3 folds in control group. At the same time,the osteogenic related gene Runx2 expression in the experimental group were significantly decreased ( P〈0.01) as com-pared with that of the control group at 1 d and 3 d,but on the 7 d this was not obvious; as to the osteogenic related genes andALP expressions in 1 d, 3 d and 7 d, the experimental group was less than the control group, and the difference was statistically signifi-cant ( P〈0.01). Conclusions : Pg-LPS can inhibit the expressions of EphA4 gene and osteogenic related genes Rux2, andin ST-2 cells in the osteogenetic differentiation.
出处
《口腔生物医学》
2017年第1期25-27,31,共4页
Oral Biomedicine
基金
国家自然科学基金资助项目(81570983)
吉林省科技厅自然科学基金资助项目(20150101076JC)