摘要
为了探讨低钾胁迫对香蕉幼苗生长和根系分泌物的影响,以及不同香蕉基因型对土壤钾活化差异。通过离位溶液培养法收集香蕉根分泌物,经低温冷冻干燥器浓缩后,进行土壤钾活化试验,利用高效液相色谱(HPLC)测定分泌物中有机酸成分及含量。结果表明,在低钾胁迫条件下,钾高效基因型香蕉幼苗鲜质量显著高于钾低效基因型。香蕉根系分泌物中的有机酸色谱图比较简单,钾低效基因型品种仅发现3种有机酸:草酸、酒石酸和柠檬酸,钾高效基因型品种发现了4种有机酸:是草酸、酒石酸、柠檬酸和琥珀酸。钾高效基因型根系有机酸、蛋白质和可溶性糖的分泌能力显著高于低效基因型,且含量变化与鲜质量变化趋势一致,总酚酸变化趋势正好相反。不同基因型香蕉根系分泌物对土壤钾素均有活化作用,其中广粉活化能力最强,各基因型间差异达极显著水平。低钾胁迫条件下,香蕉分泌大量有机物质,基因型之间差异较为明显,香蕉钾高效基因型根系有机酸的分泌能力和土壤钾的活化能力远高于低效基因型。
The objective of this study was to investigate the effects of low potassium stress on banana seedling growth and root exudates, and genotypic variation of different banana in activation of Soil potassium, Vitro bathing root system was carried out to collect root exudate and test the mobilization of Soil potassium via concentrated Cryogenic refrigeration dryer, and the content of organic acids was analyzed by high per- formance liquid chromatography （HPLC）. by using in vitro bathing root system under the condition of the water culture and low K stress. The results showed that high-efficiency genotypes grew much better than low-efficiency genotypes,Under low K stress, fresh masshigher than 75.3 % -125.4 %. Protein, total soluble sugar, total phenolic acids and organic acids in root exudates were measured. The map of iron chromatogram was relatively simple, only oxalic acid, citric acid and tartaric acid were found in root exudates of low-efficiency genotypes, but another kind of organic acid （ Succinic acid）was found in root exudates of high-efficiency genotypes. Protein, total soluble sugar and organic acids of high-efficiency genotypes were Significantly higher than low efficiency genotype, and the trends was similar with fresh mass, but total phenolic acids was opposite. Root exudates from bananas, regardless of genotype, activated potassium in the soils. However, among the four Guangfen showed the strongest effect, and the differences between high-efficiency genotypes and low-efficiency genotypes were extremely sig- nificant. Under low K stress, a large number of organic substances secreted by banana, the difference between genotypes was obvious. The high-efficiency genotypes＇ s ability of Organic acid secretion and Soil potassium activation was much higher than the low-efficiency genotypes.
作者
吴宇佳
张文
肖彤斌
符传良
吉清妹
谢良商
WU Yu-jia ZHANG Wen XIAO Tong-bin FU Chuan-liang JI Qing-mei XIE Liang-shang(Institute of Agricultural Environment and Soil, Hainan Academy of Agricultural Sciences/Scientific Observing and Experimental Station of Arable Land Conservation（ Hainan）, Ministry of Agriculture, Hainan Haikou 571100, China)
出处
《西南农业学报》
CSCD
北大核心
2017年第3期624-628,共5页
Southwest China Journal of Agricultural Sciences
基金
海南省自然科学基金项目(20153097)
海南省农业科学院农业科技创新专项(CXZX201420)
关键词
香蕉
基因型
根系分泌物
活化
有机酸
低钾胁迫
Banana
Genotype
Root exudate
Mobilization
Organic acid
K-deficiency stress
