摘要
由于头孢菌素类抗生素药物在临床上的重要性,除了对头孢菌素C(CPC)在顶头孢霉体内的生物合成途径及调控机制进行研究外,对其合成位置及相应的转运蛋白也进行了研究。基于此,本研究从顶头孢霉高产菌基因组DNA中扩增转运蛋白基因cefM序列,构建了一个cefM表达载体pYG283,将其导入顶头孢霉高产菌株中,挑选阳性转化子,并对其进行发酵培养,以观察CPC的产量。HPLC分析结果显示,转化子的CPC发酵水平均较出发菌高,其中产量最高的1#转化子较出发菌提高20%。结果表明cefM在CPC生物合成中扮演着重要角色。
Since cephalosporin antibiotics have played an important role in clinical applications, the biosynthetic pathways of cephalosporin C （CPC） have been well investigated during the fermentation of Acremonium chrysogenum. In recent years, some other regulatory proteins which have been found to be important in CPC biosynthesis, their coding genes have been discovered as well. In this study, the transporter gene cefM was amplified from the genomic DNA of a high-yield strain of A. chrysogenum and cefM expression vector pYG283 was constructed, pYG283 was then transformed into a high-yield strain of A. chrysogenum. HPLC analysis results showed that the production of CPC in the 1# transformant was improved by 20 % as compared to the original strain. It proved that cefM played an important role in the CPC biosynthesis.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2017年第3期387-392,共6页
Chinese Journal of Pharmaceuticals
基金
国家"十二五"重大新药科技专项(2011ZX09203-001-06)
上海市科委科技支撑项目(1343100204)
关键词
cefM
转运蛋白
头孢菌素C
顶头孢霉
cefM
transporter protein
cephalosporin C
Acremonium chrysogenum
