摘要
目的厄洛替尼(商品名:特罗凯)是治疗非小细胞肺癌的一种新型靶向药物,近些年来临床应用甚广,但副作用较多,其中以皮疹最为多见,且最令患者难以接受。本研究旨在观察应用特罗凯前后小鼠在表皮、病理、免疫组化等方面的改变,复制特罗凯所致皮疹的动物模型,为临床外用药物治疗皮疹提供模型。方法使用BALB/c雌性小鼠20只,随机分成4组。实验组(Ⅱ、Ⅲ、Ⅳ组)用浓度为10 g/L的特罗凯溶液按100 mg/kg灌胃,对照组(Ⅰ组)以等体积去离子水灌胃,每天1次。于给药前24 h在小鼠头颈、背、腰处脱毛,实验结束后剪取颈部、背部、腰部皮肤并观察实验组、对照组小鼠在肉眼皮肤、病理切片、免疫组化等方面的改变。结果 (1)四组小鼠之间在毛发再生天数、毛发完全再生天数、脱屑时间、出现皮疹时间、毛孔扩张数等5方面的差异存在统计学意义(P<0.01或P<0.05);(2)Ki67:四组间差异无统计学意义(P>0.05)。结论 (1)此次实验印证了许多研究者关于"EGFRIs导致的皮疹为一种炎症反应"的观点;(2)该皮疹模型可靠实用,具有可重复性,适用于"EGFRIs所致皮疹动物模型"的大批建立,可推广以供临床、试验、研究所用。
Objective Erlotinib （ Trade Name： Tarceva） is a new targeted drug for the treatment of non-small cell lung cancer （NSCLC） that has a wide clinical application in recent years, but commonly carries many side effects, among which the most common and unbearable one is rash. The aim of this study is to observe the changes of epidermis, pathology, immunohistochemistry and other aspects before and after the application of Tarceva in mice and try to copy the rash animal models caused by Tarceva and thus to provide models for the clinical topical medications of rash. Methods 20BALB/c female mice were randomly divided into four groups. The experimental group（ I1 , II1 ,IV group） was given 100 mg/kg dosage with the concentration of 10 g/L erlotinib solution by gavage, and the control group （I group）with an equal volume of deionized water by garage once daily. The hairs from the head,neck and back of each mouse were removed 24 hours prior to the administration,and at the end of the experiment, clipping the skin in the neck, back and waist , then observed differences between the experimental group and the control group in mice skin, biopsy, immunohistochemistry and the like. Results （ 1 ） There were statistically significance （P 〈 0.01 or P 〈 0.05 ） in the four groups of mice in five aspects as hair regrowth days, days of the complete regrowth of hair, desquamation time , the time of appearance of rash and the number of pore expansion;（2）Ki67 ：There were no statistically significant differenees among the four groups（P 〉 0.05）. Conclusions （ 1 ） This experiment confirms many researchers＇ point of view that ＂the rash induced by EGFRIs is an inflammatory response＂ （2） A mouse model of rash induced by FGFRIs is successfully established,and this is a reliable, practical and reproducible model which applies to a large number of establishment of ＂EGFRIs drug-induced rash in animal models＂ ,and can be popularized for clinical,experimental and institutional uses.
出处
《中国比较医学杂志》
CAS
北大核心
2017年第1期16-21,共6页
Chinese Journal of Comparative Medicine
基金
中日友好医院院内课题(2013-MS-42)
北京市科技计划首都临床特色应用研究专项(Z151100004015168)
关键词
厄洛替尼
皮疹
皮肤
病理
免疫组化
Erlotinib
Rash
Skin
Pathology
Immunohistochemistry
