摘要
目的 探讨烟曲霉孢子暴露对支气管哮喘（哮喘）大鼠肺组织糖皮质激素受体（GCR）表达的调控作用.方法 采用随机数字表法将32只Wistar大鼠随机分为4组：健康对照组（UC组）、健康对照＋烟曲霉孢子组（UC＋AF组）、哮喘组（OVA组）、哮喘＋烟曲霉孢子组（OVA＋AF组）,每组8只.OVA组和OVA＋AF组均以卵清白蛋白（OVA）致敏、激发构建哮喘模型,UC组和UC＋AF组则以生理盐水致敏并激发作为对照处理;在最后一次激发24 h后,UC＋AF组和OVA＋AF组给予烟曲霉孢子鼻腔滴入.通过气道高反应性Penh值、BALF中嗜酸粒细胞百分比（Eos%）及血清IgE水平确定哮喘模型的建立,观察各组大鼠肺组织病理变化,检测肺组织GCR mRNA和蛋白含量的变化,肺组织和全血于马铃薯葡萄糖琼脂培养基（PDA）上培养24 h后观察曲霉菌落生长情况.结果 UC＋AF组Penh值、Eos%和血清IgE水平均略高于UC组,但差异无统计学意义（均P〉0.05）;OVA组Penh值、Eos%和血清IgE水平均明显高于UC组（均P〈0.05）;OVA＋AF组在乙酰甲胆碱浓度为25和50 g/L激发时,其Penh值显著高于OVA组（均P〈0.05）,但其Eos%、血清IgE水平与OVA组差异无统计学意义（均P〉0.05）.肺组织病理检查可见OVA组和OVA＋AF组大鼠气管和肺血管周围均表现出明显的炎症细胞浸润,肺泡间隔增厚、结构破坏,平滑肌层增厚,UC组和UC＋AF组未见明显病理损害.UC＋AF组和OVA组的GCR mRNA及蛋白相对表达量明显低于UC组（GCR mRNA分别为0.93±0.15、0.65±0.10、0.72±0.22,F=10.744,P〈0.01;GCR蛋白分别为100±0、89±8、82±15,F=18.939,P〈0.01）;OVA＋AF组GCR mRNA及蛋白表达较OVA组进一步降低（GCR mRNA分别为0.52±0.08和0.72±0.22,t=2.462,P〈0.05;GCR蛋白分别为59.09±7.60和81.88±15.41,t=2.997,P〈0.05）.OVA＋AF组肺组织PDA培养半数阳性（4/8）,而UC＋AF组均为阴性（0/8）.结论 烟曲霉暴露可降低哮喘大鼠肺组织GCR的表�
Objective To study the regulative effects of Aspergillus fumigatus （ A.fumigatus ） on expression of glucocorticoid receptor （ GCR） in asthmatic rats.Methods Wistar rats were randomly divided into 4 groups：a normal control group （ UC） , a normal control with A.fumigatus group （ UC＋AF） , an OVA group （ OVA） , and an OVA with A.fumigatus group （ OVA ＋AF ） .OVA and OVA ＋AF groups were sensitized and challenged with OVA to establish asthmatic models .UC and UC ＋AF groups were given normal saline as controls .After the last challenge , OVA＋AF and UC＋AF groups were given A.fumigatus spores intranasally.Airway hyper-responsiveness, eosinophil percentage （Eos%） and serum IgE level were measured to confirm the establishment of asthmatic models .Sections of pulmonary tissue were stained with hematoxylin-eosin （ HE） and the expression of GCR mRNA and protein in lung tissues were measured by qRT-PCR and Western blot .Lung tissues and blood were plated on the potato dextrose agar （ PDA） medium and cultured for 24 h to measure the number of colony .Results The Penh value , Eos% in BALF and serum IgE level in UC＋AF group were slightly higher than those in the UC group （all the P〉0.05）.The Penh value, Eos%in BALF and serum IgE level in OVA group were significantly higher than those in the UC group （all the P〈0.05）.The Penh value in OVA＋AF group was significantly increased compared with the OVA group at the concentration of 25 g/L and 50 g/L of methacholine （ all the P〉0.05 ） .Pulmonary histology revealed that both OVA group and OVA ＋AF group showed high levels of inflammatory cell infiltration of bronchus and lung vessels , interstitial edema and smooth muscle thickening , while the UC and UC ＋AF groups were normal.Compared with the UC group, the expressions of GCR mRNA and protein in UC＋AF group and OVA group were decreased significantly （ GCR mRNA in UC, UC＋AF and OVA group were 0.93 ±0.15, 0.65 ±0.10, 0.72 ±0.22,respectively,F=10.744,P〈0.01;
作者
周霞
党亚洁
王桂芳
金先桥
Zhou Xia Dang Yajie Wang Guifang Jin Xianqiao(Department of Respiratory Medicine, Huashan Hospital Affiliated to Fudan University, Shanghai 200040, Chin)
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
2017年第3期182-187,共6页
Chinese Journal of Tuberculosis and Respiratory Diseases
基金
上海市科委基础研究领域重点项目（12JC1407501）
关键词
曲霉菌
烟
哮喘
受体
糖皮质激素
Aspergillus fumigatus
Asthma
Receptors
glucocorticoid
