摘要
通过对水稻抗稻瘟病基因Pi2/9位点上已克隆的功能基因及非功能位点序列的相互对比,鉴定到Pi2特异的核苷酸差异,开发了基于PCR技术的Pi2基因的插入/缺失(insert-deletion,InDel)标记,能有效地将Pi2与其它抗性等位基因及感病等位基因区分开。利用Pi2基因的分离群体进行标记选择与接种鉴定,结果表明Pi2-InDel标记能精确地选择Pi2基因。用Pi2-InDel标记对20份水稻品种和育种亲本进行分子检测,该标记能准确地将抗性基因分离开来。这为筛选水稻稻瘟病抗性种质资源,以及抗性标记辅助抗病育种提供了便利。
The molecular markers, designated as Pi2-InDel, for detecting rice alleles of Pi2 has been successfully developed through sequence comparison strategy. This specific marker Pi2-InDel can obviously distinguish Pi2 gene from others mapped on Pi2/9 locus. With Pi2-InDel marker, 20 representative cultivars and breeding materials can be diagnosed. Moreover, this marker can accurately selected out the Pi2 gene, which facilitates the screening and identification of resistant germplasm to rice blast, and molecular marker assisted breeding for rice blast resistance.
作者
杨立明
纪剑辉
周颖君
方继朝
刘永锋
罗玉明
Yang Liming Ji Jianhui Zhou Yingjun Fang Jichao Liu Yongfeng Luo Yuming(School of Life Science, Huaiyin Normal University, Jiangsu Key Laboratory for Eco-Agficultural Biotechnology around Hongze Lake, Jiangsu Col laborative Innovation Center of Regional Modem Agriculture ＆ Environmental Protection, Huaian, 223300 Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014)
出处
《分子植物育种》
CAS
CSCD
北大核心
2017年第2期594-598,共5页
Molecular Plant Breeding
基金
国家自然科学基金项目(31640006
31400169)
江苏省自然科学基金项目(BK20140454)
淮安市科技计划项目(HAC2014012)
江苏省"区域现代农业与环境保护"协同创新中心项目
江苏省高校"青蓝工程"项目共同资助
