摘要
通过ISSR分子标记技术,对采自河北石家庄、保定、邢台、邯郸、张家口的11个杠柳(Periploca sepium Bunge)样品进行遗传多样性分析。结果表明,从100条通用引物中共筛选出34条适用于杠柳ISSR研究的多态性引物;对11个供试样品进行PCR检测,进一步筛选出8条多态性好、稳定性高的引物,共检测出79个ISSR位点,多态性位点63个,占79.7%;遗传相似系数(GS)变化范围在0.454 5~0.854 5,8对多态性引物多态信息含量值变化范围为0.747~0.884,平均为0.827;11份供试样品聚类分为三大类,聚类相对复杂,呈一定的地域相关性;ISSR分子标记适用于杠柳遗传多样性研究,且杠柳遗传多样性丰富。
ISSR makers was used to analyze the genetic diversity of 11 Periploca sepium Bunge samples from Shijiazhuang,Handan,Xingtai,Zhangjiakou,Baoding.The results showed that 34 polymorphic primers were selected from 100 general primers for ISSR analysis.11 samples were tested for PCR,and 8 primers with good polymorphism and stability were screened out,79 ISSR loci were amplified by 8 primers,among which 63 loci were polymorphic,accounting for 79.7%.Genetic similarity coefficient varied in the range of 0.454 5~0.854 5.the change range of PIC values of polymorphic primers was0.747~0.884,the average was 0.827.11 samples were clustered into 3 groups,and the cluster was relatively complex,the accessions were significantly related to the geographical.ISSR molecular markers were applied to the study of genetic diversity of Periploca sepium Bunge,and the genetic diversity was rich.
作者
狄魁颖
黄大庄
贾艳晶
张显国
王伟
霍腾达
DI Kui-ying HUANG Da-zhuang JIA Yan-jing ZHANG Xian-guo WANG Wei HUO Teng-da(College of Landscape and Travel,Agricultural University of Hebei, Baoding 071000,Hebei, China Highway Administration Bureau of Hebei Province, Shijiazhuang 050000,China Great Wall Motor Co., Ltd., Baoding 071000,Hebei,China Luanping County Propaganda Department,Chengde 068250, Hebei,China)
出处
《湖北农业科学》
2017年第3期570-574,共5页
Hubei Agricultural Sciences
基金
河北农业大学项目(1508003)
