摘要
采用土培试验,以越冬稻、R1088(籽粒Cd低积累)和IR34582、太粳558(籽粒Cd高积累)4个水稻品种为试验材料,研究不同水稻品种在Cd0(不加Cd)、Cd5(5 mg·kg^(-1))和Cd25(25 mg·kg^(-1))胁迫下对Cd的吸收、转运及其分配特征,以期为培育镉低积累水稻品种提供科学依据。结果表明,(1)Cd胁迫下,Cd低积累水稻品种根Cd含量显著高于Cd高积累水稻品种,而地上部Cd含量却显著低于Cd高积累水稻品种;同时,其TF_(根-茎)和TF_(茎-叶鞆)也显著低于Cd高积累水稻品种。(2)Cd在各组织亚细胞中的含量表现为细胞壁>可溶部分>细胞膜>细胞器。Cd胁迫下,Cd低积累水稻品种根系细胞壁(F1)、细胞器(F2)、细胞膜(F3)和可溶部分(F4)Cd含量均显著高于Cd高积累水稻品种,而茎、叶鞘和叶中F1、F2、F3和F4Cd含量却显著低于Cd高积累水稻品种。(3)不同水稻品种各组织间转移系数与根系Cd亚细胞分布比例相关性分析表明,Cd5处理中,TF_(根-茎)分别与F1和F4分配比例呈极显著负相关和显著正相关,相关系数分别为-0.93 1(P<0.01)和0.702(P<0.05);Cd25处理中,TF_(根-茎)与F1和F4分配比例呈极显著负相关和极显著正相关,相关系数分别为-0.908和0.786(P<0.01)。综上所述,Cd胁迫下水稻各组织Cd含量显著增加,且Cd含量和TF_(根-茎)在Cd高低积累水稻品种间存在显著差异,根系细胞壁和可溶部分在Cd从根部向上转运的过程中起着重要作用。
Here the characteristics of uptake,transfer and distribution of Cd in in histocyte of different rice varieties were studied in a pot experiment,provide a scientific basis for cultivating low Cd accumulation rice varieties.Rice varieties included Overwintering Riceand R1088（low Cd accumulated in grain）,IR34582 and toojing 558（high Cd accumulated in grain）.Cadmium concentrations were 0 mg·kg-（-1）（Cd0）,5 mg·kg-（-1）（Cd5） and 25 mg·kg-（-1）（Cd25）.The results showed：（1） The Cd concentrations in root of were significantly higher than those of high Cd accumulated.However,the Cd concentrations in shoot of low Cd accumulated rice variety were significantly lower than those of high Cd accumulated,the same to TF_（root-stem） and TF_（stem-leaf sheaths）.（2） The content of Cd in the central Asia of tissues was expressed as the cell wallsoluble partcell membrane organelle.Cd contents of the root cell wall（F1）,organelle（F2）,cell membrane（F3） and soluble fraction（F4） were in low accumulation rice varieties were significantly higher than those of high Cd accumulated.Howere,the Cd content of Fl,F2,F3 and F4 in stem,leaf sheath and leaf were significantly lower than that of Cd high accumulation of rice varieties.（3） The correlation analysis of the transfer coefficient and the distribution of Cd subcellular distribution in different rice varieties was indicated that TF_（root-stem） was negatively correlated with the proportion of F1 distribution under the Cd5 treatment（R-2=0.-931,P〈0.01）,and TF_（root-stem） was significantly positively correlated with the proportion of F4 under the same treatment（R-2=0.702,P〈0.05）.TF_（root-stem） was extremely negatively correlated with the proportion of F1 distribution under the Cd25 treatment（R-2=-0.908,P〈0.01）,and TF_（root-stem） was extremely positively correlated with the proportion of F4 under the same treatment（R-2=0.786,P〈0.01）.In summary,the content of Cd in rice tissues increased signif
出处
《生态环境学报》
CSCD
北大核心
2016年第12期2014-2020,共7页
Ecology and Environmental Sciences
基金
国家科技支撑计划项目(2012BAD14B18)
四川省科技支撑计划项目(2013NZ0028)
关键词
水稻
CD胁迫
亚细胞分布
细胞壁
可溶部分
rice
Cd stress
subcellular distribution
cell wall
soluble fraction
