摘要
【目的】明确新疆主要农区烟粉虱生物型分布及其对常用杀虫剂的抗性水平,为新疆烟粉虱有效防治及抗性治理提供科学依据。【方法】采用线粒体细胞色素氧化酶I基因(mt COI)酶切扩增多态性序列(CAPs)技术进行烟粉虱的生物型鉴定,利用浸叶法和着卵浸渍法测定新疆伊宁、和田、莎车、吐鲁番和昌吉烟粉虱种群对11种常用杀虫剂的抗性水平。【结果】生物型鉴定显示Q型烟粉虱在测试的5个田间种群中均有分布,和田烟粉虱是以B型为优势种的混合型种群,伊宁、莎车、昌吉和吐鲁番种群均为Q型;抗性监测表明吐鲁番种群对吡虫啉的抗性达到57.67倍的高抗水平,伊宁种群仅对吡丙醚具有抗性。吐鲁番、莎车、和田、昌吉种群对吡虫啉、噻虫嗪、呋虫胺和啶虫脒产生了低到高水平的抗性(RR 5.53~57.67倍),对吡蚜酮+异丙威具有低到中等水平的抗性(RR 5.14~31.92倍);吐鲁番和昌吉种群对阿维菌素及溴氰虫酰胺都产生了中等水平的抗性(RR 31.67~32.73倍,RR 10.93~29.57倍),对氟啶虫胺腈为低到中等水平的抗性(9.7~11.66倍);莎车、吐鲁番及伊宁种群对吡丙醚产生了低至中等水平的抗性(7.96~31.33倍)。【结论】测试的5个烟粉虱种群以Q型为主,B型仅有少量分布;不同烟粉虱种群对常用药剂的抗性水平不同,对吡虫啉抗性水平最高,应根据当地的抗性水平进行针对性的抗性治理。
[ Objective] This project aims to make clear the biotype distribution of Bemisia tabaci in the main agricultural areas of Xinjiang and commonly used pesticides resistance level in order to provide a scientific basis for scientific prevention and pesticides resistance management. [ Method ] Mitochondrial cytochrome oxidase I gene （mtCOI） enzyme sequence amplification polymorphism （CAPs） technology was applied to i- dentify the whiteflies biotype. The resistances to 11 commonly used insecticides in Yining, Hotan, Shache, Turpan and Changji were determined by the methods of leaf - dipping and egg impregnation leaf - dipping. [ Result] Biological identification showed that Q type was distributed in the 5 tested field populations. Bemisia tabaci in Hotan was a mixed type with B dominant species. Those found in Yining, Shaehe, Changji and Turpan belonged to type Q. Resistance monitoring showed that the high level of resistance to imidacloprid resistant population in Turpan reached 57.67 times. The population of Yining was only resistant to Imidaeloprid. Those populations in Turpan, Shache, Hotan and Changji resulted in from low to high levels of resistance to imida- cloprid, thiamethoxam, acetamiprid and dinotefuran （RR was 5.53 -57.67 times） and led to from low to middle levels of resistance to pymetrozine ＋ isoprocarb （RR 5.14 -31.92 times） ; Populations in Turpan and Changji displayed moderate level resistance to avermectins and cyantraniliprole （RR 31.67 -32.73 times, 10.93 -29.57 times） and to low to moderate level resistance to sulfoxaflor （RR 9. 7 - 11. 66 times）. Shache, Yining and Turpan populations exhibited low to moderate level resistance to pyriproxyfen （RRT. 96 - 31.33 times）. [ Conclusion] The tested five Bemisia tabaci field populations＇biotypes mainly belong to Q biotype with a small amount of B biotype. Different Bemisia tabaci biotype shows different resistance levels, among which, the highest level of resistance is to the lmidacloprid. Resistance management should be
作者
贾尊尊
王小武
付开赟
丁新华
郭文超
吐尔逊.阿合买提
姜卫华
卡德.艾山
JIA Zun - zun Wang Xiao - wu FU Kai - yun DIN Xin - hua GUO Wen - chao Tuerxun Ahemaiti JIANG Wei -hua Kader Aishan(College of Agronomy, Xingfiang Agricultural University, Urumqi 830052, China College of Agronomy, Shihezhi University, Shihezi Xinjiang 832003, China Key Laboratory of Intergraded Management of Harmful- Crop Vermin of China North - western Oasis, Ministry of Agriculture, P. R. China/Research Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences, Ururaqi 830091, China Institute of Microorganism Application in Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China Xinjiang Academy of Agricultural Sciences, Turpan Xinjiang 838000, China)
出处
《新疆农业科学》
CAS
CSCD
北大核心
2017年第2期304-312,共9页
Xinjiang Agricultural Sciences
基金
自治区科技援疆项目"烟粉虱抗性分子检测技术研发"(201591140)
国家重点研发计划"生物安全关键机技术研发"重点专项"主要入侵生物的动态分布与资源库建设"(2016YFC1202101)~~
关键词
烟粉虱
生物型
抗药性
Bemisia tabaci
biotype
resistance to pesticides
