摘要
通过富集培养的方法,从长期使用异菌脲的土壤中筛选到一株可以以异菌脲为唯一碳源的菌株CQH,它可以在112 h完全降解100 mg/L的异菌脲。结合其生理特征和16S rRNA基因序列分析,将菌株CQH鉴定为Arthrobacter sp.CQH。在单因素实验的基础上,考察培养基中不同p H、培养温度和培养基中Na Cl浓度对菌株CQH生长的影响,通过Box-Behnken实验设计和响应面分析法探索菌株CQH最适培养条件。结果显示,菌株CQH的最适培养条件为:p H为7.3,培养温度36℃,培养基中Na Cl浓度为0.5%,在此条件下培养11 h后,菌株CQH的菌悬液在600 nm处吸光度为0.582。
By enrichment culture method, a bacterial strain CQH capable of using iprodione as the sole carbon source and energy source for its growth was isolated from the soil. It could completely degrade 100 mg/L of iprodione within 112 h. In combination with the phylogenetic analysis of 16S rRNA gene and the physiologic ＆ biochemical characteristics, strain CQH was identified as Arthrobacter sp.CQH.Based on the single factor experiment,Box-Benhnken experimental design and response surface analysis method were used to optimize the culture condition of strain CQH.The optimum culture condition of strain CQH was that pH was 7.3, culture temperature 36 ℃ and NaCl concentration 0.5% .Under these conditions, the optical density of bacterial suspension at 600 nm was 0.582 after culturinz 11 h.
作者
曹礼
舒润东
石文红
张亮
庞健
丁莉
雷玉明
CAO Li SHU Run- dong SHI Wen- hong ZHANG Liang PANG Jian DING Li LEI Yu- ruing(College of Agriculture and Biotechnology, Hexi University, Zhangye 734000, China Key Laboratory of Hexi Corridor Resourses Utilization of Gansu, Hexi University,Zhangye 734000, China)
出处
《食品工业科技》
CAS
CSCD
北大核心
2017年第6期168-173,178,共7页
Science and Technology of Food Industry
基金
河西学院大学生科技创新项目
自然科学基金项目(31560029)
河西走廊特色资源利用重点实验室项目(XZ1401)
