摘要
通过Fmoc固相合成法,以Rink Amide MBHA树脂为载体、Fmoc-Asp-OAll为起始原料、DIEA/HCTU为缩合体系,按照crourorb A1的氨基酸序列由C端向N端依次偶联后,在树脂上实现环合,切割后经制备型RP-HPLC纯化,得到纯度为98%的抗肿瘤环九肽crourorb A1,总收率34%。
Using Fmoc solid-phase polypeptide synthesis （SPPS） with Rink Amide MBHA resin as a solid support, Fmoc-Asp-OAll as a starting material, and DIEA/HCTU as a condensation system, crourorb A1 was synthesized according to its amine acid sequence form C-terminal to N-terminal by sequential coupling and cycli- zation on the resin. After cleavage, the anti-tumor cyclic nanopeptide was purified by preparative RP-HPLC with a final purity of 98% and yield of 34%.
出处
《化学与生物工程》
CAS
2017年第3期31-34,共4页
Chemistry & Bioengineering
基金
四川省高校科研创新团队项目(13TD0028)
国家大学生创新创业训练计划项目(201513705024)
