摘要
目的:了解单硝酸异山梨酯对自发性高血压大鼠(SHR)肺组织NO、i NOS、IL-1及IL-6水平的影响与意义。方法:14周龄Wistar和SHR雄性大鼠各20只,分别随机分成W0、W1、S0、S1四组,每组各10只。其中,W0和S0组饲喂生理盐水和普通饲料,W1和S1组饲喂单硝酸异山梨酯和普通饲料。喂养12周后,取大鼠肺组织检测NO、i NOS、IL-1、IL6的浓度。结果:W1组NO、i NOS、IL-1、IL6与WO组相比,水平偏高且差异均有统计学意义(P<0.05);S1组NO、i NOS、IL-1、IL6与SO组相比,水平偏高且差异均有统计学意义(P<0.05);W1、S1组NO、i NOS水平均与IL-1、IL-6呈正相关。结论:(1)单硝酸异山梨酯会导致正常大鼠肺组织NO、i NOS、IL-1、IL6的显著增加,即预示着慢性炎症的存在;(2)长期饲喂单硝酸异山梨酯后,可能会导致高血压大鼠机体炎症因子的增加,加重肺组织的炎症状态,造成肺组织的损害。
Objective To investigate the effect of isosorbide mononitrate on the levels of NO, iNOS, IL-1 and IL-6 in lung tissue of spontaneously hypertensive rats （SHR） . Methods Fourteen-week-old Wistar and SHR male rats were randomly divided into the W0, W1, SO and S1 group, with 10 rats ineach group. Rats in the W0 and SO group were fed with the normal saline and the ordinary food, rats in the W1 and S1 group were fed with isosorbide mononitrate and the ordinary food. Twelve weeks later, levels of NO, iNOS, IL- 1 and IL-6 in rat lung tissue were detected. Results Compared with the W0 group, levels of NO, iNOS, IL- 1 and IL-6 were significantly increased in the W1 groups （P 〈 0.05, respectively）. Compared with the SO group, levels of NO, iNOS, IL-1 and IL-6 were significantly increased in the S1 group （P 〈 0.05, respectively）. In the W1 and S1 group, levels of iNOS and NO were positively correlated with IL-1 and IL-6. Conclusion 1. Isosorbide mononitrate may lead to increases of NO, iNOS, IL-1 and IL6 in lung tissue of Wistar rats, which indicates the presence of chronic inflammation. 2. Longterm feeding of isosorbide mononitrate may lead to increases of inflammatory factors in SHR rats, contributing to the inflammatory state in rats.
作者
赵文飞
李宏云
白冰
ZHAO Wenfei LI Hongyun BAI Bing(Fifth Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Chin)
出处
《实用医学杂志》
CAS
北大核心
2017年第4期547-550,共4页
The Journal of Practical Medicine
