摘要
研究3种不同地理起源的葡萄种质资源河岸葡萄‘河岸(♀)’、燕山葡萄‘燕山-1’和欧洲葡萄‘红地球’的抗旱性差异,为进一步挖掘葡萄的抗旱基因提供依据。以3种不同葡萄的试管苗为材料,在培养基中加入PEG-6000模拟干旱胁迫,研究其形态变化和6项生理生化指标的变化,进而评价其抗旱性。结果表明,干旱胁迫后,3种葡萄试管苗叶片边缘开始出现干枯的时间不同,‘河岸(♀)’、‘红地球’和‘燕山-1’分别出现于3、12h和27h;随着干旱胁迫时间的延长,受害程度最大的是‘河岸(♀)’,其次是‘红地球’,‘燕山-1’仅有轻微的伤害。叶片中脯氨酸含量总体呈上升趋势,胁迫72h时,‘燕山-1’分别是‘红地球’和‘河岸(♀)’的1.14倍和4.84倍;POD、CAT和SOD含量呈先升高后下降趋势,72h时,‘燕山-1’的POD含量分别是‘红地球’和‘河岸(♀)’的6.2倍和49.2倍,CAT含量分别是‘红地球’和‘河岸(♀)’的0.44倍和1.47倍,SOD含量分别是‘红地球’和‘河岸(♀)’的1.95倍和1.05倍;超氧阴离子自由基含量(O_2~·)呈先升高后下降趋势,72h时,‘燕山-1’的O_2~·含量分别是‘红地球’和‘河岸(♀)’的1.9倍和3.0倍;MDA含量呈先升高后下降趋势,72h时‘燕山-1’分别是‘红地球’和‘河岸(♀)’的1.4倍和1.26倍。综合形态变化及各项生理生化指标的变化,以‘燕山-1’的抗旱性最强,其次是‘红地球’,抗旱性最差的是‘河岸(♀)’。
The objective of this study was to examine the differences in drought resistance of three grape- vines from different geographic origins, including Vitis yeshanensis accession‘ Yanshan-1’ （Y）, Vitis ri- paria accession‘ He＇an （♀）’（H） and Vitis vinifera cv. ‘Red Globe’ （R）. The plantlets of the three were used as materials, the changes of morphology, proline, MDA, the activities of protective enzymes and super- oxide anion free radicals contents in the blades were investigated under the stress of PEG-6000. The occur- rence of the dry spots in the blades was different among the three： the spots appeared 3,12 and 27 hours after the stress for H,R,and Y,respectively. With the increase of drought stress time,H was injured most- ly,followed by R,and Y was slightly injured. The proline contents in the blades of the three increased with time. Seventy-two hours after the stress,the proline content in the blades of Y was 1.14 and 4.48 folds to R and H,respectively. The activities of POD,CAT and SOD initially presented an upward trend but all declined later. Seventy-two hours after the stress,the POD activity of Y was 6.2 and 49.2 folds to R and H, the CAT activity of Y was 0.44 and 1.47 folds R and H,and the SOD activity of Y was 1.95 and 1.05 folds to R and H, respectively. The superoxide anion free radicals content initially increased, and then decreased. At 72 h after the stress,the superoxide anion free radicals content of Y was 1.9 and 3.0 folds to R and H, respectively. The MDA content initially increased, but declined later. At 72 h after stress, the MDA content of Y was 1.4 and 1.26 folds to R and H,respectively. Considering morphological changes and the changes of the physiological and biochemical indexes comprehensively,Y demonstrated the highest drought resist- ance,followed by R and H.
作者
薛加楠
崔小月
张琪
吴梦晓
张剑侠
XUE Jia-nan CUI Xiao-yue ZHANG Qi WU Meng-xiao ZHANG Jian-xia(College of Horticulture ,State Key Laboratory of Crop Stress Biology in Arid Areas ,Key Laboratory of Northwest Horticulture Plant Germplasm and Application of Ministry of Agriculture, Northwest A＆F University ,Yangling, Shaanxi 712100, China)
出处
《西北林学院学报》
CSCD
北大核心
2017年第2期136-142,共7页
Journal of Northwest Forestry University
基金
国家科技支撑计划子课题(2013BAD02B04-06)
