摘要
采用水培试验方法,研究了重金属胁迫对小麦水稻DNA胞嘧啶甲基化的影响。结果表明,和对照相比,Cu2+、Cd2+、Hg2+能明显使小麦和水稻地上部叶片和幼穗中的5-甲基胞嘧啶(5mC)的含量增高,即引起叶和穗DNA高甲基化。0.1、0.5mmol·L-1的Cu2+和Cd2+胁迫能使小麦根系DNA中的5-甲基胞嘧啶的百分含量显著高于对照,而各供试浓度的Hg2+以及1.0mmol·L-1的Cu2+和Cd2+胁迫则造成小麦根系DNA中的5-甲基胞嘧啶的百分含量显著低于对照,使DNA低甲基化。
Protein synthesis in leaf of crops wa s inhibited obviously by heavy metal stress.The reason may be due,at least in part,to change of DNA methylation pattern ca used by heavy metal stress.Comparin g with the control,when wheat and rice were treated by heavy metal(Cu 2+ ?Cd 2+ ?Hg 2+ )stress,it was significant that cont ent of 5-methyl cytosine in the DNA of leaf and ear in wheat or rice was increased.When wheat was tr eated by 0.1,0.5mmol·L -1 Cu 2+ and Cd 2+ stress,the content of 5-methyl cytosine in DNA of root in wheat was much higher th an that in control.When it was treate d by 0.1,0.5,1.0mmol·L -1 Hg 2+ or 1.0mmol·L -1 Cu 2+ and Cd 2+ stress,the content of 5-methyl cytosine in r oot DNA of wheat was markedly lower th an that in control.The results suggested that heavy metal s tress could,to a different extent ,induce the modification of cytosine methylation in DNAof crops.
出处
《农业环境保护》
CSCD
北大核心
2002年第4期301-305,共5页
Agro-Environmental Protection
