摘要
Objective： To explore the molecular biological mechanism of acupuncture in intervening visual deprivation. Methods： Forty-eight 2-week old Wistar rats were randomly divided into a normal group, a model group, and 6 acupuncture groups （group C1： acupuncture at the unaffected side in early stage; group C2： acupuncture at the affected side in early stage; group DI： acupuncture at the unaffected side in mid-stage; group D2： acupuncture at the affected side in mid-stage; group El： acupuncture at the unaffected side in late stage; group E2： acupuncture at the affected side in late stage） by the random number table, 6 rats in each group. Rats in the normal group didn＇t receive any interventions. The rat model of deprivation amblyopia was established by unilateral eyelid suture in the model group and each acupuncture group After successful modeling, rats in model group didn＇t receive any treatments; rats in the acupuncture groups received acupuncture intervention which began respectively on the 3rd, 12th and 21st day after modeling. Pattern visual evoked potential （P-VEP） and N-methy D-aspartatreceptor-1 （NMDAR1） mRNA expression in visual cortex area 17 were detected at the end of acupuncture intervention in each group. Results： After the intervention, the P-VEP waveform was significantly changed, with a significantly delayed Pzoo value （P〈0.01） and significantly decreased amplitude of N45-Ploo in the model group versus the normal group （P〈O.01）; the P-VEP waveform was significantly improved, with obviously earlier P10o （P〈0.01） and increased amplitude of N4s-Ploo （P〈O.05） in each acupuncture group versus the model group. The improvement effect of acupuncture on the P-VEP waveform in group C1 and C2 was more significant than that in group D1, D2, E1 and E2. The expression of NMDAR1 mRNA of the rat visual cortex area 17 in the model group was significantly lower than that in the normal group （P〈0.01）; and the expression of NMDAR1 mRNA in the visual cortex are
目的:探讨针刺干预视觉剥夺效应的分子生物学机制。方法:采用随机数字表法将48只2周龄的Wistar大鼠随机分为正常组、模型组和6个针刺组[早期针刺患侧组(C1)、早期针刺健侧组(C2)、中期针刺患侧组(D1)、中期针刺健侧组(D2)、晚期针刺患侧组(E1)和晚期针刺健侧组(E2)],每组6只。正常组不予任何处理;模型组和各针刺组采用单侧眼睑缝合的方法建立剥夺性弱视动物模型,造模成功后,模型组不予任何治疗;早期、中期、晚期针刺组分别于造模后第3天、12天、21天开始针刺治疗。治疗结束后检测各组大鼠图形视觉诱发电位(P-VEP)和视皮层17区N-甲基-D-天门冬氨酸受体1(NMDAR1)m RNA的表达。结果:模型组大鼠P-VEP波形较正常组有明显改变,表现为P_(100)时值明显延迟(P<0.01),N_(45)-P_100幅值显著降低(P<0.01);治疗后,各针刺组大鼠P-VEP波形较模型组均有显著改善,表现为P_(100)时值明显提前(P<0.01),N45-P_(100)幅值明显升高(P<0.05);并且早期针刺对P-VEP波形的影响大于中期和晚期针刺。模型组大鼠视皮层17区NMDAR1 mRNA的表达较正常组明显降低(P<0.01);而治疗后各针刺组大鼠视皮层17区NMDAR1 m RNA表达较模型组均显著提高(P<0.05);其中早期针刺对NMDAR1 mRNA表达水平的影响大于中期和晚期针刺;而早期针刺患侧穴位对NMDAR1 mRNA表达水平的影响优于针刺健侧穴位(P<0.05);中期、晚期针刺患侧穴位与健侧穴位对NMDAR1 mRNA表达水平的影响差异无统计学意义(P>0.05)。结论:单眼剥夺后大鼠P-VEP波形出现异常改变,视皮层17区NMDAR1 mRNA表达减少。敏感期内针刺对单眼剥夺大鼠异常的P-VEP波形和视皮层17区NMDAR1 mRNA低水平表达均具有明显的调节作用,并且敏感期内早期治疗是取得疗效的关键。
作者
Zhu Tian-tian
Chen Cheng
Yan Xing-ke
朱田田;陈程;严兴科;杨燕萍(译)(School of Acupuncture and Tuina,Gansu University of Chinese Medicine,Lanzhou 730000,China;Health Management Center,Daqing Hospital of Traditional Chinese Medicine,Daqing 163311,China;不详)
基金
supported by National Natural Science Foundation of China,No.81260560~~
