摘要
目的观察调节小分子GTP结合蛋白Rab27表达对人膀胱癌细胞中细胞周期蛋白(Cyclin)、磷酸化黏着斑激酶抗体(p-FAK)、核因子κB抑制蛋白(p-IκB)表达的影响。方法取对数生长期人膀胱癌细胞系BIU-87(Rab27低表达细胞系)用Rab27质粒转染,人膀胱癌细胞系5637(Rab27高表达细胞系)用Rab27干扰质粒转染。采用Western blotting法检测转染前后BIU-87及5637细胞Rab27、Cyclin D1、Cyclin E、p-FAK(Tyr925)、p-FAK(Tyr576/577)及p-IκB。结果与Rab27质粒转染前比较,转染后BIU-87细胞Rab27的相对表达量升高,细胞Cyclin D1、Cyclin E、p-FAK(Tyr925)、p-FAK(Tyr576/577)及p-IκB相对表达量均升高(P均<0.05)。与Rab27干扰质粒转染前比较,5637细胞Rab27的相对表达量降低(P均<0.05),细胞Cyclin D1、Cyclin E、p-FAK(Tyr925)、p-FAK(Tyr576/577)及p-IκB相对表达量均降低(P均<0.05)。结论转染Rab27质粒的BIU-87细胞Cyclin D1、Cyclin E、p-FAK(Tyr925)、p-FAK(Tyr576/577)及p-IκB的表达升高。转染Rab27干扰质粒的5637细胞Cyclin D1、Cyclin E、p-FAK(Tyr925)、p-FAK(Tyr576/577)及p-IκB的表达降低。Rab27可能通过调节NF-κB、FAK信号通路的相关蛋白表达来影响膀胱癌细胞周期。
Objective To observe the effect of regulating the expression of small molecule GTP binding protein Rab27 on the cell cycle protein（ Cyclin）,phosphorylated focal adhesion kinase antibody（ p-FAK） and nuclear factor κB（ pIκB） of human bladder cancer cells. Methods Rab27 plasmid and Rab27 siRNA were transfected into BIU-87 and 5637 cells in logarithmic phase,respectively. The levels of Rab27,Cyclin D1,Cyclin E,p-FAK（ Tyr925）,p-FAK（ Tyr576 /577） and p-IκB were analyzed by Western blotting in the bladder cancer BIU-87 and 5637 cells before and after transfection. Results Compared with the Rab27 plasmid transfected BIU-87 cells before transfection,the relative expression of Rab27 was increased,and the expression of Cyclin D1,Cyclin E,p-FAK（ Tyr925）,p-FAK（ Tyr576 /577） and p-IκB was increased（ all P 〈 0. 05）. After the Rab27 interference plasmid was transfected into 5637 cells,the relative expression of Rab27 was decreased（ all P 〈 0. 05）,and Cyclin D1,Cyclin E,p-FAK（ Tyr925）,p-FAK（ Tyr576 /577） and p-IκB relative expression was decreased（ all P 〈 0. 05）. Conclusion After the Rab27 plasmid is transfected into BIU-87 cells,the expression of Cyclin D1,Cyclin E,p-FAK（ Tyr925）,p-FAK（ Tyr576 /577） and p-IκB is increased. After the Rab27 interference plasmid is transfected into 5637 cells,the expression of Cyclin D1,Cyclin E,p-FAK（ Tyr925）,p-FAK（ Tyr576 /577） and p-IκB is decreased. Rab27 may affect the cell cycle of bladder cancer by regulating the expression of NF-κB and FAK signaling pathway.
作者
刘嘉
刘春来
宫雪
薛东炜
刘屹立
王平
LIU Jia LIU Chunlai GONG Xue XUE Dongwei LIU Yili WANG Ping(The Fourth Affiliated Hospital of China Medical University, Shenyang 110032, China)
出处
《山东医药》
CAS
北大核心
2017年第7期9-12,共4页
Shandong Medical Journal
基金
辽宁省科学技术基金资助项目(2015225009)
