摘要
土壤微生物作为生态系统的重要组成部分,在生态系统的物质转化和能量流动等生物地球化学循环过程中起着重要作用.本文以亚热带典型森林为研究对象,通过设置隔离穿透水的干旱处理、样地建设影响的干扰处理,以及自然状态下的空白对照处理,利用磷脂脂肪酸(Phospholipid Fatty Acid,PLFA)分析法,研究了干旱环境对森林生态系统土壤微生物生物量及群落结构的影响.结果表明:不同处理方式对土壤温度影响差异不显著,但干旱显著降低了土壤湿度.与对照组相比,干扰处理下土壤微生物生物量与群落结构没有显著变化,干旱处理轻微显著降低了3个土层土壤微生物总PLFAs、细菌和真菌PLFAs含量(P<0.1).干旱对i15:0和16:0两种表征细菌的脂肪酸含量没有显著影响,但对a15:0,i16:0,16:12OH和18:1ω7c等4种表征细菌的脂肪酸含量影响明显.土壤微生物总PLFAs含量与土壤含水量在干旱处理下显著正相关,而在干扰和对照处理下呈现负相关关系.这些结果表明,干旱影响了森林生态系统土壤微生物生物量及群落结构,并且土壤水分是引起干旱条件下微生物生物量变化的关键因素.
Soil microbes are essential components in terrestrial ecosystem, which play crucial roles in biogeochemical cycling,such as material transformation and energy flow.Our study focused on effects of drought on soil microbial biomass and community structure by throughfall displacement experiment（TDE）treatment with drought,disturbance,and control treaatments in a subtropical evergreen broadleaf forest,Ningbo,Zhejiang.Results showed that soil temperature did not change with drought and disturbance treatments,while drought significantly decreased soil moisture.Compared with control treatment,disturbance had no significant effects on soil microbial biomass and community structure,but drought reduced total microbial biomass,bacteria and fungi biomass with marginal significance（P〈0.1）.In addition,although drought did not significantly affect bacteria biomass with markers of i15：0and 16：0,it significantly decreased those with markers of a15：0,i16：0,16：12OH and 18：1ω7c.Furthermore,total soil microbial biomass exhibited the positive correlation with soil water content under drought,but negative ones occurred in control and disturbance treatments.Therefore,drought affected soil microbial biomass and community structure in the subtropical forest,and soil water content is the key factor in regulating these changes under drought.
作者
聂园园
周贵尧
邵钧炯
周灵燕
刘瑞强
翟德苹
周旭辉
NIE Yuanyuan ZHOU Guiyao SHAO Junjiong ZHOU Lingyan LIU Ruiqiang ZHAI Deping ZHOU Xuhui(Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science, Fudan University, Shanghai 200438, China School of Ecological and Environmental Sciences, East China Normal University, Shanghai 200062, China School of Life Sciences, Anhui Agricultural University, He f ei , Anhui Province, 230036, China)
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
2017年第1期97-105,共9页
Journal of Fudan University：Natural Science
基金
国家自然科学基金(31370489)
