摘要
目的了解肇庆市2014年流行的登革热病毒I型毒株E基因序列进化情况,寻找爆发来源,为本地登革热的防控提供科学依据。方法收集肇庆市2014年登革热病例急性期血清进行病毒分离培养和基因扩增测序,构建系统发生树,进行生物信息学分析。结果从36份登革病例急性期血清标本中分离到20株登革I型病毒,在进化树上可分为3个主要的分支,与当年广州、中山和佛山分离到的登革病毒I型毒株最为接近。结论肇庆市2014登革热疫情在生物学上存在多个来源,可能是周边临近地区病例输入引起的本地爆发。
Objective To understand the E gene characterization of Dengue virus serotype I strains of Zhaoqing City in 2014 and look for the source of dengue fever outbreak, so as to provide the evidence for the control of dengue fever in the future. Methods The serum samples of dengue fever cases in the acute phase were collected for realtime RT-PCR test and virus isolation. The data of E gene sequences of the virus strains were obtained for phylogenetic analysis.Results A total of 20 strains of Dengue virus serotype I were isolated in 36 serum samples of the dengue fever cases. The result of phylogenetic analysis showed that the 20 strains of Dengue virus can be divided into 3 branches by the E gene,which was biologically closest to the virus isolated from Guangzhou, Zhongshan and Foshan cities in the same year. Conclusion The local outbreak of dengue fever in Zhaoqing City in 2014 might be caused by imported cases from the cities with dengue epidemic nearby, and it is informed that there might be 3 sources of this outbreak, according to the result of phylogenetic analysis of E gene.
作者
朱颖梅
谭海芳
苏乐斌
谭翰清
程洁萍
ZHU Ying- mei TAN Hal-fang SU Le-bin et al(Center for Disease Control and Prevention of Zhaoqing, Zhaoqing 526060, Guang- dong, Chin)
出处
《中国校医》
2017年第2期134-136,共3页
Chinese Journal of School Doctor
关键词
登革热病毒/分离和提纯
基因型
进化
分子
Dengue Virus/isolation and purification
Genotype
Evolution
Molecular
