摘要
[目的]通过废弃资源的再利用研究可替代的新型生物燃料——丁醇的发酵培养基。[方法]对菌株Clostridium acetobutylicum CGMCC 1.0134进行紫外诱变和磁场与Fe^(2+)共同诱变,获得1株丁醇产量高和稳定性好的优良突变株Clostridium acetobutylicum UM-80,采用不同的废弃原料考察该突变菌株的发酵性能,并筛选出合适的性价比高的培养基。[结果]突变株UM-80发酵产丁醇和总溶剂(丙酮、丁醇、乙醇)分别为9.04、17.95 g/L,较原始菌株分别提高了5.12%、4.12%。单独的蕨根是较好的丁醇发酵原料,当蕨根醪质量分数为15%时发酵液中丁醇浓度最高为5.50 g/L。当高山被孢霉与蕨根共同发酵时发酵液中丁醇最高产量为6.50 g/L。[结论]蕨根与高山被孢霉的复合培养基是较好的生物丁醇发酵培养基。
[ Objective] The aim was to study better fermentation medium for new type biofuel butanol through recycling waster resources. [ Method] Clostridium acetobutylicum UM-80, a mutant with high butanol yield and good stability, was obtained by ultraviolet mutagenesis and co-mutagenesis with Fe^2＋ by Clostridium acetobutylicum CGMCC 1. 0134. Then the different fermentation materials were used to study the fermentation performance of the mutant strains, and the suitable medium with high cost performance was found out. [ Result ] The yield of butanol and total solvent （acetone, butanol, ethanol） were 9.04 and 17.95 g/L, respectively, which were 5.12% and 4.12% higher than those of the original strain. The fern root was the best raw material for the fermentation of butanol; when the mass fraction of fern root was 15% , the concentration of butanol in fermentation broth was 5.50 g/L. When the co-fermentation of Mortierella alpina and fern root, the highest yield was 6.50 g/L. [ Conclusion] The co-fermentation of Mortierella alpina and fern root is better fermentation medium for butanol.
出处
《安徽农业科学》
CAS
2017年第5期1-3,共3页
Journal of Anhui Agricultural Sciences
基金
合肥物质科学技术中心方向项目培育基金(2014FXCX006)
安徽省淮南市科技计划项目(2014A15)
中国科学院合肥分院等离子体物理研究所科学基金(DSJJ-15-YY02)
关键词
丙酮丁醇梭菌
复合诱变
废弃物原料
丁醇
Clostridium acetobutylicum
Compound mutagenesis
Waste raw material
Butanol
