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饲料中添加N-羟甲基蛋氨酸钙对泌乳期奶牛血清生化指标的影响 被引量:4

Effects of N-hydroxymethyl methionine calcium in dietary on serum biochemical parameters of lactating dairy cows
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摘要 为研究饲料中添加不同比例N-HMM-Ca对泌乳期奶牛血清生化指标的影响,试验选用胎次、产奶量和泌乳天数相近身体健康的荷斯坦奶牛30头,完全随机区组分为5组,每组6头。对照组饲喂基础日粮;试验组分别饲喂在基础日粮中以干物质基础添加0.15%、0.30%、0.75%和1.50%的N-HMM-Ca,预试期14d,正试期42d。结果表明:1)泌乳期奶牛日粮中添加0.75%N-HMM-Ca使血清中谷丙转氨酶(ALT)和谷草转氨酶(AST)浓度显著升高。2)在第14天时,1.50%组甘油三酯(TG)浓度显著高于其他各处理组(P<0.01)。血清中高密度脂蛋白(HDL)水平有所提高,但差异不显著(P>0.05);随着N-HMM-Ca添加量的增加血清中非酯化脂肪酸(NEFA)的浓度逐渐降低(P<0.05);对β-羟丁酸(β-OHB)来说,随着日粮中N-HMM-Ca添加量的增加浓度逐渐升高(P<0.05)。3)0.15%、0.30%和0.75%组血清IgA浓度呈现增加的趋势,而且在第42天时尤为显著(P<0.01)。4)在第28天时,泌乳期奶牛日粮中分别添加0.15%和0.30%的N-HMM-Ca使过氧化氢酶(CAT)浓度显著高于其他各处理组(P<0.01)。综上所述,泌乳期奶牛日粮中添加0.15%的N-HMM-Ca有利于改善机体健康。 To study the effects of different N-hydroxymethyl methionine calcium (N-HMM-Ca) supplementation levels on serum's biochemical indexes of lactating dairy cows.Thirty lactating Holstein dairy cows,which have similar parity,milk yield and lactating stage,were randomly divided into 5 groups with 6 cows in each group.The control group was fed with basal diet,the test groups were fed with 0.15%,0.30%,0.75% and 1.50% levels of N-HMM-Ca of basal diet (on DM basis),respectively.The experiment lasted up to 56 d,including 14 d of adaptation period and 42 d of experimental period.The results showed that:1) Supplemented 0.75% dosage of N-HMM-Ca on the diet improved the serum ALT and AST concentrations;2) On the 14th day of experimental period,after supplemented 1.50% N-HMM-Ca the triglyceride (TG) concentration was significantly increased (P〈0.01).Serum high-density lipoprotein (HDL) and β-hydroxy butyric acid (β-OHB) concentration were increased with N-HMM-Ca supplementation,nevertheless,serum non-esterified fatty acid (NEFA) was decreased linear;3) Serum IgA concentration was significantly increased in 0.15% and 0.30% groups on 42th day (P〈0.01);4) On 28th day,serum catalase (CAT) concentration was significant higher in 0.15% and 0.30% level than in other treatments (P〈0.01).In conclusion,0.15% N-HMM-Ca supplementation in the diet was beneficial to improve the health of dairy cows.
出处 《中国农业大学学报》 CAS CSCD 北大核心 2017年第3期94-101,共8页 Journal of China Agricultural University
基金 江苏省高校优势学科建设工程项目(PAPD) 上海市科学技术委员会国内科技合作项目(14395810100)
关键词 荷斯坦奶牛 N—HMM—Ca 血清酶 免疫指标 抗氧化指标 cows N-HMM-Ca biochemical serum enzyme immune index antioxidant indices
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