摘要
目的:探讨miR-581对结直肠癌SW620细胞增殖能力的影响。方法:分别用携带miR-581基因的慢病毒颗粒(LV-miR-581-GFP)和miR-581 mimics(miR-581)转染人结肠癌细胞株SW620作为实验组;用对照组病毒颗粒(LV-GFP)和对照组mimics(Vector)转染SW620细胞株作为阴性对照组。用实时荧光定量PCR(qRT-PCR)检测各组细胞中miR-581的mRNA表达水平;CCK8和克隆形成实验检测细胞增殖能力和克隆形成能力的变化。结果:实验组SW620细胞中miR-581表达较对照组明显增高(P<0.05);CCK8和克隆形成实验显示过表达实验组细胞的增殖及克隆形成能力较对照组明显增强(P<0.05)。结论:miR-581对结直肠癌SW620细胞的增殖具有促进作用。
Objective：To investigate the role of miR-581 overexpression on the proliferation of human colorectal cancer cell line SW620. Methods： The expression group, colorectal cancer SW620 cells were transfected with recombinant lentivirus vector （LV-miR- 581 ） and miR-581 mimics（ miR-581 ） ,the negative control group were transfected with negative control lentiviral vector （LV-GFP） and negative control mimics （vector）. The mRNA expression of miR-581 was identified by qRT-PCR. Proliferation of the cells were detected by CCK8 assary and colony forming assary. Results： The expression of miR-581 at mRNA significantly increased in LV-miR-581 group compared with control groups were detected by qRT-PCR （ P〈0. 05 ）. Up-regulation of miR-581 markedly enhanced human colorectal cancer SW620 cells proliferation than those in the cells transfected with control vector （ P〈0. 05 ）. Conclusion ： Forced expression of miR-581 accelerates the proliferation of colorectal cancer SW620 cells.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2017年第2期252-255,共4页
Chinese Journal of Immunology
基金
国家自然科学基金项目(81560407)资助
