摘要
目的以胡桃醌类成分转移率为指标,通过静态吸附和解吸附筛选最佳树脂,建立青龙衣胡桃醌类成分的富集工艺。方法比较4种不同极性的大孔吸附树脂对青龙衣胡桃醌类成分的静态吸附与解吸性能,筛选出AB8树脂为最佳树脂,优化其工艺参数。结果最佳工艺参数为:上样液浓度0.0760 g/ml、上样p H2.0、上样流速3BV/h,洗脱浓度70%、洗脱体积3BV、洗脱流速2BV/h,径高比1∶10。工艺优化后,青龙衣中胡桃醌类成分的含量由1.026%提升到11.08%,转移率为72.08%。结论 AB8型大孔树脂能有效地富集纯化青龙衣胡桃醌类成分。
Objective To optimize the best macroporous resin through static adsorption and desorption and establish enrichment process of juglone-constituents from Qinglongyi with the transfer rate of juglone constituents as the index. Methods The static adsorption and desorption of four different resins with different polarity to juglone-constituents from Qinglongyi were compared. AB8 resin was selected as the best resin. Results The craft parameters of the best resin were optimized,and the results were as follows：initial concentration was 0.0760 g/ml,the p H was 2.0,the flow rate of adsorption was 3BV/h,the elution concentration of ethanol was70%,the elution volume was 3BV,the desorption flow rate was 2BV/h,and the ratio of diameter to height was 1∶10. After the process of optimization,the content of juglone-constituents from Qinglongyi increased from 1.026% to 11.08% and the transfer rate was72.08%. Conclusion The AB8 macroporous resin could be used to enrich and purify juglone-constituents from the Qinglongyi.
出处
《国际药学研究杂志》
CAS
CSCD
北大核心
2017年第1期60-64,共5页
Journal of International Pharmaceutical Research
基金
北京中医药大学创新创业项目(201510026048)
北京中医药大学自主选题中青年教师资助项目(2013-JYBZZ-JS-137)
榆林现代特色农业新技术研究与集成示范(2015CXY-13)
