摘要
目的:探讨血小板低下对过继性树突状细胞(DC)在体内迁移模式的影响。方法:腹腔注射抗CD41单克隆抗体以建立血小板低下小鼠模型。将带报告基因的骨髓来源DC经脚垫和静脉两种途径输注至血小板低下和正常小鼠体内,用活体成像技术监测DC在小鼠体内的迁移和组织内分布情况。结果:经抗GPⅡb抗体处理组小鼠体内80%以上血小板被清除。DC经脚垫回输72 h后,在血小板低下组迁移到腘窝淋巴结(PLN)和腹股沟淋巴结(ILN)的比例分别为(0.32±0.02)%和(0.02±0.01)%;对照组上述两部位内为(0.27±0.15)%和(0.02±0.02)%。两组之间无统计学差异(P>0.05)。经静脉输注DC 72 h后,血小板低下组和对照组小鼠组织内的分布均主要集中在脾脏、肝脏引流淋巴结、肺脏和肝脏等,且分布比例无统计学差异。结论:小鼠体内血小板低下至不足20%时,对经皮下和静脉两种途径输注的DC在体内迁移和组织分布无明显影响。
Objective：To investigate the effect of thrombocytopenia on the migration patterns of adoptive dendritic cell（DC） in vivo.Methods;The mouse model of thrombocytopenia was established by intraperitoneal administration of anti-CD41 mAb MWReg30.Mouse bone marrow（BM）-derived DC were injected into thrombocytopenia mouse by footpad infusion and intravenous infusion.The DC migration and distribution pattern were detected by bioluminescence imaging.Results-.More than 80%platelets were cleared in the experimental group which was infused with anti-CD41 antibody.At 72 h after injection,the percentage of injected DC that migrated from footpad to popliteal lymph nodes（PLNs） and inguinal lymph nodes（ILNs） were（0.32 ±0.02）%and（0.02 ±0.01）%in experimental group,and（0.27 ±0.15）%and（0.02 ±0.02）%in control group,respectively.Statistic data showed that there was no statistical difference between these 2 groups（P〉 0.05）.The issue distribution pattern of intravenously injected DC between experimental group and control group were not distinctly different,and large amounts of injected DC accumulated in the spleen,liver draining lymph-nodes lungs and liver.Conclusion;Thrombocytopenia has not a distinct effect on the migratory capacity and tissue distribution of DC by either footpad or intravenous injection.
出处
《中国实验血液学杂志》
CAS
CSCD
北大核心
2017年第1期171-175,共5页
Journal of Experimental Hematology
基金
国家自然科学基金资助项目(81401369
81371806)
