摘要
目的应用UFLC-Q-TOF/MS对黄芪-丹参药对中的化学成分进行鉴定。方法采用Thermo BDS C18(3μm,150mm×2.1mm)色谱柱,以0.1%甲酸水-0.1%乙腈为流动相进行梯度洗脱,流速为0.3mL/min,柱温40℃,进样量5μL。离子源为电喷雾离子源(ESI),负离子模式下采用飞行时间质谱采集数据,全扫描质量范围为m/z 100~1 500。依据保留时间、精确分子量、二级质谱裂解碎片,结合对照品比对初步鉴定化合物。结果从黄芪-丹参药对中初步鉴定或推断出28个化合物,其中丹参中15个化合物,黄芪中11个化合物,另有2个未知化合物。包括皂苷类、有机酸类、黄酮类和醌类化合物。结论该UFLC-Q-TOF/MS可全面、快速分析黄芪-丹参药对中的化学成分,研究结果为对其进行活性成分研究和质量控制提供了一定参考。
OBJECTIVE To identify the main chemical components in herbal pair：Astragali Radix and Salviae Miltior- rhizae by UFLC-Q-TOF/MS. METHODS Thermo BDS C18 （3μm, 150 mm × 2.1 mm） column was employed for the separa- tion with acetonetrile-water （0.1 % formic acid） as mobile phase using a gradient elution program. The flow rate was set as 0. 3 mL/min, the temperature of column was 40 ℃ with injection volume of 5 μL. Electrospray ionization （ESI）-Q-TOF/MS in negative ion mode was performed to obtain the MS spectra with full mass scan m/z ranged from 100 to 1 500. Chemicals were identified based on the retention time, high-resolution mass information, MS/MS fragmentation behaviors and standards. RE- SULTS 28 components were identified or tentatively characterized in herbal pair：Astragali Radix and Salviae Miltiorrhizae, including 15 components in Salviae Miltiorrhizae, 11 compounds in Astragali Radix, and 2 unknown compounds. And these chemicals could be classified as triterpenes, organic acids, flavonoids and quinines. CONCLUSION This method can rapidly and accurately identify the main compounds in herbal pair：Astragali Radix and Salviae Miltiorrhizae, which can help us for the quality control and further phytochemical studies of Astragali Radix and Salviae Miltiorrhizae. in traditional Chinese medicine.
出处
《南京中医药大学学报》
CAS
CSCD
北大核心
2017年第1期93-96,103,共5页
Journal of Nanjing University of Traditional Chinese Medicine
基金
国家自然科学基金青年基金(81403386)
江苏省自然科学基金青年基金(BK20161078)
中国中医科学院江苏分院课题(JSBY1316)
