摘要
目的探讨绿茶多酚(GTPs)对正常膳食大鼠肾皮质Nampt/SIRT3表达量及氧化应激水平的影响。方法雄性Wistar大鼠(体质量160~180g)20只,经适应性喂养1w后,随机分为正常对照组(CN)和绿茶多酚干预组(GTPs),每组10只。对照组自由摄食标准饲料,绿茶多酚干预组按照体质量和进食量将绿茶多酚[200mg/(kg·bw)]添加至饲料。18w末断头处死大鼠并收集肾脏组织及全血;采用试剂盒测定血清胆固醇、甘油三酯和血糖等指标;免疫组化方法检测肾皮质4-羟基壬烯酸(4-HNE)水平;采用Western-blotting方法测定Nampt和SIRT3的蛋白表达水平。结果 GTPs组大鼠血低密度脂蛋白胆固醇(LDL-C)和肾皮质4-HNE水平低于对照组(P<0.05);Nampt和SIRT3蛋白表达水平高于对照组(P<0.05)。结论绿茶多酚干预可以上调肾皮质NAMPT和SIRT3表达,进而降低氧化应激水平。
Objective To explore the influence of green tea polyphenols （GTPs） on the expression of Nampt/SIRT3 and renal oxidative stress level.Methods Twenty male Wistar rats were randomly divided into two groups（10 rats in each group）：the control group （CN）,fed on a standard chow and the GTPs treated groups（GTPs）,fed on the standard chow supplemented with GTPs. After 18 weeks, the rats were sacrificed. Blood lipids, MDA, 4-HNE, Nampt and SIRT3 were detected by kits, immunohistochemical analysis, and Western blotting respectively.Results The levels of blood LDL-C and renal cortex 4-hydroxy nonene acid （4-HNE） in the GTPs group were significantly lower （P〈0.05） than in the control group. The expression levels of nicotinamide phosphoribosyltransferase （Nampt） and Sirtuin 3 （SIRT3） in rat kidney were higher in the GTPs group compared to the CN group （P〈0.05）.Conclusion GTPs can enhance the expression levels of NAMPT and SIRT3, and thus may reduce oxidative stress in the kidney and contribute to the renoprotection.
出处
《营养学报》
CAS
CSCD
北大核心
2017年第1期81-85,共5页
Acta Nutrimenta Sinica
基金
国家自然科学基金(No.81302423)
