摘要
目的对乙型肝炎病毒表面抗原/梅毒螺旋体抗体(Hepatitis B virus surface antigen/Treponema pallidum antibody,HBs Ag/TP)双联金标试剂的结果进行评价,探讨无偿献血者献血前进行HBs Ag/TP双联金标试剂快速筛查的可行性。方法采用HBs Ag/TP双联金标试剂试纸条法、HBs Ag-ELISA和TP-ELISA法对880份无偿献血者血液标本进行平行检测评估;对百色市中心血站2012~2015年无偿献血者血液标本HBs Ag/TP常规筛查记录资料进行统计分析。结果 880份无偿献血者血液标本双联金标试纸条检测HBs Ag/TP结果与ELISA法检测结果符合率为99.77%;采用HBs Ag/TP双联金标试剂进行血液初筛后TP复检阳性率为0.72%,TP复检阳性率比较,差异有统计学意义(χ2=85.350,P<0.001)。结论采用HBs Ag/TP双联金标试剂进行无偿献血前筛查,可有效减少不合格血液的采集以及降低血液报废率,节约血液资源,值得广泛推广应用。
Objective To evaluate results of double gold-labeled reagent in Hepatitis B virus surface antigen/Treponema pallidum antibody,so as to discuss feasibility of rapid screening of double gold-labeled reagent in unpaid blood donors before blood donation.Methods HBs Ag/TP double gold labeled reagent test strip method,HBs Ag-ELISA and TP-ELISA methods were put into use in parallel detection evaluation of blood samples from 880 blood donors.HBs Ag/TP routine screening data of blood samples from blood donors from 2012 to 2015 in Baise City were statistically analyzed.Results The coincidence rate of the test results of double gold labeled reagent with the methods of HBs Ag/TP and ELISA from 880 blood samples was 99.77%,while the reexamination of TP positive rate was 0.72% after adopting HBs Ag/TP double gold labeled reagent for blood screening.The positive rate of TP recheck was compared,and difference was statistically significant( χ2= 85.350,P<0.001).Results HBs Ag/TP double gold labeled reagent for blood donation before screening can effectively reduce collection of unqualified blood and reduce blood condemnation rate,it can also save blood resources.Therefore,it is worthy of wide application.Conclusion The use of HBs Ag/TP double gold labeled reagent for blood donation before screening can effectively reduce the collection of unqualified blood and reduce the rate of blood scrap,as well as it can save blood resources.Therefore,it is worth wide application.
出处
《右江医学》
2017年第1期88-90,共3页
Chinese Youjiang Medical Journal
