摘要
利用富集培养的方法,将采自葫芦岛市某畜禽养殖堆肥场周边土壤样品进行分离纯化并筛选出土霉素优势降解菌株(命名为YH1),通过形态观察,菌落边缘不整呈灰白色中心凸起似草帽状,采用16S rDNA基因序列分析该菌株为短波单胞菌属(Brevundimonas sp.),并对培养条件与降解特性进行优化,该菌株在温度30℃、pH值为6.0、土霉素质量浓度300mg/L、装液量80mL时,降解率最高可达到70.34%.此外,添加不同的碳源或氮源和金属离子对菌株降解能力均有影响,其中加入2g/L可溶性淀粉,麦芽糖时可提高菌株的降解率,添加1g/L的Fe^(3+)和Cu^(2+)时可提高菌株的降解率;而Cd^(2+)、Mn^(2+)可明显抑制其降解率.
An oxytetracycline-degrading strain from the soil of poultry breeding farms in Huludao City was isolated by enrichment culture method and its degradation conditions were optimized. The strain named YH 1 was identified based on the morphology observation; the colony morphology was gray, irregular margin, and the center was raised like a straw hat. By 16S rDNA gene sequence, the preliminary identification suggested that the strain belonged to Brevundimonas sp. When the temperature was 30 ℃, pH was 6.0, and mass concentration of oxytetracycline was 300 mg/L , the degradation rate of the bacterial strain was up to 70. 34%. Adding different and metal ions had an impact on the degradation of strains; when 2 g/L soluble starch and maltosewere added; the degradation rate of the strain was increased; when 1 g/L of Fe3 and Cu^2 was added, the degradation rate of the strain was increased; while Cd^2 and Mn^2 could inhibit the degradationrate.
出处
《沈阳大学学报（自然科学版）》
CAS
2017年第1期21-25,共5页
Journal of Shenyang University：Natural Science
基金
国家自然科学基金资助项目(31470552)
辽宁省教育厅资助项目(L2012405)
关键词
土霉素
微生物降解
条件优化
16S
RDNA
oxytetracycline
microbial degradation
condition optimization
1 6S rDNA
