摘要
目的研究不同浓度马尾松针提取物(PMNE)对牙本质脱矿的理化性能影响,为PMNE抑制根面龋的研究提供实验依据。方法将离体牙根部牙体组织块分别放到去离子水(DDW)、氟化钠(Na F)、4%PMNE、8%PMNE及12%PMNE等5个组,每组10个试件(n=10)。在37℃条件下,各组试件经实验溶液、酸性缓冲液(p H5.0)和中性缓冲液(p H7.0)组成的p H循环,一天3次,共8天。用显微硬度计测定脱矿前后的牙体表面显微硬度差值(△SMH),电感耦合等离子体原子发射光谱仪测定并计算酸性缓冲液中的钙离子释出率(CDR)。结果 PMNE组CDR均显著低于DDW组,显著高于Na F组(P<0.05),且8%PMNE组CDR显著低于4%PMNE组(P<0.05);各PMNE组△SMH均显著低于DDW组(P<0.05),且8%PMNE组△SMH略高于Na F组,但无统计学意义(P>0.05),两者均显著低于4%和8%PMNE组。结论 PMNE溶液可抑制根面牙本质脱矿,减缓其表面硬度的降低,且8%浓度组效果较佳。
Objective To study the effect ofpinus massoniana needle extract （PMNE） at different concen- trations on the physical and chemical properties of root dentin, and to explore the mechanism which PMNE inhibits root surface caries. Methods Root tooth blocks were randomly divided into 5 groups, and immersed into distilled deionized water （DDW）, sodium fluoride （NaF）, 4%PMNE, 8%PMNE,or 12% PMNE （n=10 for each group）. All samples were treated with pH cycling for 8 days, consisting of experimental solution, acidic buffer, and neutral buf- fer, at a frequency of 3 times per day. The D-value of surface micro-hardness （ A SMH） before and after pH cycling was determined using Vickers hardness tester, and the calcium depletion rate （CDR） in acid buffer was measured by inductively-coupled plasma atomic emission spectrometer. Results The CDR of all PMNE groups was lower than that of DDW group and was higher than that of NaF group （P〈0.05） , and the CDR of 8% PMNE group was lower than that of 4% PMNE group （P〈0.05） . The △ SMH of 4%, 8%, and 12% PMNE groups was lower than that of DDW group （P〈0.05） , but△ SMH was similar between the 8% PMNE group and the NaF group （P 〉0.05）, both of which had lower △ SMH than 4% and 12% PMNE groups. Conclusions PMNE solution can inhibitdemineralization of root teeth and slow down the decrease of surface hardness, and 8% PMNE has the best effect.
出处
《口腔材料器械杂志》
2017年第1期11-16,24,共7页
Chinese Journal of Dental Materials and Devices
基金
国家自然科学基金(81641150)
陕西省科技厅社会发展项目(编号:2015SF242)
关键词
马尾松针提取物
根面龋
牙本质
脱矿
Pinus massoniana needle extract
Root caries
Dentin
Demineralization
