摘要
为了探明来自川西北的6份短芒披碱草(Elymus breviaristatus)种质材料的苗期抗旱性差异,采用室内盆栽法对种质进行为期21d的干旱处理,分别于停止浇水第0、7、14和21天取植株叶片,研究了不同水分胁迫条件下各种质的相对含水量(RWC)、相对电导率(REC)、脯氨酸(Pro)含量、叶绿素(Chl)含量、丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性7个生理特征指标的变化,并采用隶属函数法对6份短芒披碱草种质材料的抗旱性进行综合评价。结果表明,干旱胁迫对不同抗旱短芒披碱草种质材料的各生理指标有显著的影响(P<0.05),其各表型值与对照差异显著(P<0.05),且各种质间差异显著(P<0.05)。随着干旱胁迫时间的延长,6份短芒披碱草材料的相对含水量和叶绿素含量呈显著下降趋势(P<0.05),而丙二醛含量、相对电导率、脯氨酸含量和POD活性表现出上升趋势,SOD活性呈现先上升后下降的趋势。由隶属函数分析7个生理指标得到供试材料苗期抗旱性强弱顺序依次为205176>205133>红10-5>204290>红6-5>205104。
Drought tolerance of six Elymus breviaristatus accession samples from the Northwest Plateau of Sichuan Province was investigated over 21 days using a water-controlled potting experiment. Relative water content （RWC）, relative electric conductivity （REC）, content of proline （Pro）, content of chlorophyll （Chl）, content of malondialdehyde （MDA）, activity of superoxide dismutase （SOD）, and peroxidase （POD） of leaves were measured in the control （CK, without drought stress）, and on the 7th, 14th, and 21st days after drought stress treatment, respectively. Additionally, drought resistance of germplasms was evaluated using subordinate function analysis. Our results showed that drought stress had significant effects on physiological indices （P〈0.05）, and significant differences in phenotypic values were observed between drought treatments and the control （CK） （P〈0.05）. There was also a significant difference detected between the different accession samples （P〈0.05）. With the extension of drought stress time, RWC and Chl followed a declining trend. By contrast, MDA, REC, Pro and the activity of POD followed a significant rising trend. The activity of SOD increased at first and then decreased. Based on the subordinate function analysis of seven physiological characteristics, the rank order for drought resistance among these E. breviaristatus accessions were as follows： 205176〉205133〉 Red 10-5〉204290〉Red 6-5〉205104.
出处
《草业科学》
CAS
CSCD
北大核心
2017年第1期101-111,共11页
Pratacultural Science
基金
四川省科技支撑计划(2013ZC1635)
国家科技支撑计划(2012BAD13B06)
关键词
短芒披碱草
苗期
抗旱性
生理特性
隶属函数
Elymus breviaristatus
seedling stage
drought resistance
physiological characteristics
subordinate function
