摘要
目的:探讨肿瘤蛋白p53(TP53)基因rs1042522位点单核苷酸多态性与男性不育的关系。方法:采用病例-对照研究,收集南京地区特发性男性不育患者380例(病例组),有生育史的健康男性398例(对照组);并将病例组分为无精子症组(n=140)和少精子症组(n=240)。用Sequence Mass Array技术检测TP53基因rs1042522位点的基因型,通过Logistic回归分析该位点多态性与男性不育的相关性。结果:病例组与对照组前向运动精子百分率[(10.38±5.57)%vs(42.55±9.57)%]、精子浓度[(13.13±24.96)×106/ml vs(77.34±49.24)×106/ml]、T[(14.07±5.36)nmol/L vs(11.89±4.50)nmol/L]、FSH[(16.80±18.20)U/L vs(4.55±7.17)U/L]存在显著性差异(P<0.05)。经Logistic回归分析,未发现各基因型与男性不育有统计学意义的相关性,亚组分析也未发现相关性。结论:TP53基因rs1042522位点的单核苷酸多态性与男性不育无显著相关性。
Objective ： To investigate the correlation of the single nucleotide polymorphism （SNP） rs1042522 of the tumor pro- tein p53 （ TP53 ） gene with the risk of male infertility. Methods ： This case-control study included 380 male patients with idiopathic infertility and 398 normal fertile men as controls from the Nanjing area. We genotyped the SNP rs1042522 of the TP53 gene by Se- quence Mass Array and analyzed the correlation of the SNP with male infertility using the logistic regression model. Results ： Corn-pared with the normal controls, the patients with idiopathic infertility showed significantly decreased sperm concentration （ [ 77.34 ± 49.24 ] vs [ 13.13 ± 24.96 ] x 10^6/ml）, percentage of progressively motile sperm （ [42.55 ±9.57 ] vs [ 10.38 ±5.57 ] % ）, serum testosterone level （ [ 14.07 ± 5.36] vs [ 11.89± 4.50 ] nmol/L）, and follicle-stimulating hormone level （ [ 16.80 ± 18.20 ] vs [ 4.55±7.17] U/L） （P 〈0.05） but no statistically significant differences in other parameters. No correlation was observed between the SNP frequencies and male infertility and similar results were found in the subgroups of the cases. Conclusion ： SNP rs1042522 of the TP53 gene is not significantly correlated with the risk of male infertility.
出处
《中华男科学杂志》
CAS
CSCD
北大核心
2017年第2期142-146,共5页
National Journal of Andrology
基金
江苏省科技厅省级科技专项(BM2015020)
南京军区南京总医院院管题(2015046)~~
