摘要
将PCR-变性梯度凝胶电泳(DGGE)与传统梯度浓度富集手段相结合,从不同氨氮浓度养殖污水中筛选高活性氨氮降解菌株。结果表明,经过不同氨氮浓度的驯化,初筛得到22株菌株,分别测定其氨氮降解能力,最终得到3株氨氮降解能力较强的菌株,分别命名为ZZC-3、ZZC-4和ZZC-14。经16S r DNA鉴定后,分别为戈登氏菌属、反硝化菌属和红球菌属细菌。PCR-DGGE结果表明,反硝化杆菌属的微生物在富集液中的含量最高,因此选择ZZC-4为目标菌株。经过污水降解效果验证,添加ZZC-4菌株的处理在144 h后水中氨氮和化学需氧量降解率分别达到90.8%和94.7%,显示该菌株具有良好的应用开发前景。
In the present study,strains able to efficiently degrade ammonia in livestock waste water were screened with traditional enrichment selection method combined with PCR-denaturing gradient gel electrophoresis（ DGGE）.Twenty-two strains were screened out after domestication under different ammonia concentrations. Among them,3strains exhibited relatively higher ammonia degradation abilities,and were named as ZZC-3,ZZC-4 and ZZC-14,respectively,which were identified as Gordonia sp.,Denitrobacter sp. and Rhodococcus sp. by 16 S r DNA sequence analysis. The PCR-DGGE results confirmed that the Denitrobacter sp. had the highest concentrations in the enriched solution of waste water. Therefore,strain ZZC-4 was selected as the target strain. After incubation for 144 h,the treatment with strain ZZC-4 reached the highest degradation efficiency,which was 90. 8% for ammonia nitrogen and94. 7% for COD,respectively.
出处
《浙江农业学报》
CSCD
北大核心
2017年第2期286-291,共6页
Acta Agriculturae Zhejiangensis
基金
浙江省重点研发计划项目(2015C03013)
浙江省农科院青年人才培养项目
