摘要
通过探讨银杏叶聚戊烯醇纳米乳液(GBP)的平均粒径对其自身抗菌增效作用的影响,以及考察最小抑菌浓度(MIC)和分级抑菌浓度(FIC);同时研究亚抑菌浓度条件下,聚戊烯醇纳米乳液与抗生素联合作用的时间-杀菌曲线,研究了银杏叶聚戊烯醇纳米乳液与氨苄西林(A)、环丙沙星(C)、硫酸庆大霉素(G)、红霉素(E)和多粘菌素B硫酸盐(P)这5种抗生素的协同抗菌作用。结果显示:聚戊烯醇乳液配伍抗生素的抑菌圈直径随着乳液平均粒径增大而减小,由此推断聚戊烯醇纳米乳液可能具有提高聚戊烯醇自身的增效能力。聚戊烯醇纳米乳液与硫酸庆大霉素混合对金黄色葡萄球菌具有协同抗菌作用(FIC指数为0.5),混合样抑菌圈值为(25.8±0.1)mm,MIC值为33 mg/L。聚戊烯醇与硫酸庆大霉素混合可以有效地提高对金黄色葡萄球菌的抗菌效果并延长抗菌时间。
The synergistic antibacterial effects of Ginkgo biloba leaves polyprenols nanoemulsion （GBP） combined with five types of antibiotics,i.e., ampicillin （A）, ciprofloxacin （C）, gentamicin sulfate（G）, erythromycin（E）, and polymyxin B sulfate （P）, were determined through analyzing the average diameter of the inhibition halos, minimal inhibitory concentration （MIC）, fractional inhibitory concentration （FIC） index and the time-killing curves under sub-MIC conditions. The results showed that the antibacterial effect of polyprenols nanoemulsion combined with the antibiotics enhanced with the increase of the average diameter of polyprenols nanoemulsion. The antibacterial effect of polyprenols could be improved by nanoemulsion. The polyprenol combined with gentamicin sulfate had synergistic antibacterial effect against Staphylococcus aureus with the FIC index of 0.5, the diameter of the inhibition halos of （25.8±0.1）mm, and the MIC value of 33.0 mg/L. The time-killing curves showed that the antibacterial effect and the antibacterial time could be increased after polyprenols mixed with gentamicin sulfate.
出处
《林产化学与工业》
EI
CAS
CSCD
北大核心
2017年第1期81-86,共6页
Chemistry and Industry of Forest Products
基金
中国林科院林业新技术所基本科研业务费专项资金(CAFINT2014C10)
江苏省自然科学青年基金项目(BK20150070)
江苏省林业三新工程项目(LYSX[2014]09)
关键词
银杏
聚戊烯醇
纳米乳液
抗生素
抗菌作用
Ginkgo biloba
polyprenol
nanoemulsion
antibiotic
antibacterial activity
