摘要
目的:探讨非编码RNA MIR155HG对人肺癌A549细胞增殖、迁移和侵袭能力的影响及机制。方法:过表达或敲减MIR155HG后,MTS检测A549细胞生长的变化,流氏细胞仪检测细胞周期。Transwell迁移和侵袭实验检测过表达或敲减MIR155HG后,A549细胞迁移和侵袭能力的变化。过表达MIR155HG后,定量PCR检测A549细胞中mi R-155-5p的表达。结果:MTS法显示,转染72 h和96 h后,过表达MIR155HG组细胞吸光度(absorbance,A)值分别为(2.47±0.14)和(3.13±0.15),均较对照组[(2.09±0.12)(72 h)和(2.50±0.13)(96 h)]增加(P=0.006,P=0.027);敲减MIR155HG组细胞在48、72和96 h的OD值分别为(1.69±0.15),(1.87±0.09)和(2.24±0.16),较对照组[(2.04±0.06)(48 h),(2.43±016)(72 h)和(2.88±0.11)(96 h)]均降低(P=0.006,P=0.006和P=0.004);敲减MIR155HG组的A549细胞G0/G1期比例为63.93%,与对照组(54.32%)相比,增加了9.61%;迁移实验结果显示,过表达MIR155HG组的穿膜细胞数为(31.67±3.51)个,与对照组(12.67±2.51)相比增加明显(P=0.002)。敲减MIR155HG组的A549细胞的穿膜细胞数为(13.67±3.21)个,与对照组(36.00±4.58)相比明显减少(P=0.002)。侵袭实验结果显示,过表达MIR155HG组细胞穿膜细胞数为(42.33±7.02)个,与对照组(15.67±3.51)相比明显增加(P=0.004)。敲减MIR155HG组穿膜细胞数为(15.00±3.60)个,与对照组(39.00±4.36)相比明显减少(P=0.002)。定量PCR显示,过表达MIR155HG后,mi R-155-5p的表达较对照组增加的倍数为(17.99±2.42)(P=0.000)。结论:MIR155HG能增强A549细胞的增殖、迁移和侵袭能力,其发挥作用的机制可能是通过产生mi R-155-5p来实现。
Objective：To investigate the effects and mechanism of MIR155 HG on proliferation,cell cycle,migration and invasion of A549 human lung cancer cells. Methods：MTS assay,flow cytometry,migration assay and invasion assay were applied to study the changes of cell proliferation,cell cycle,migration and invasion after overexpression or knock down of MIR155 HG in A549 cells. The expression level of mi R-155-5p was detected after overexpression of MIR155 HG. Results：MTS results showed that the absorbance（A）value was higher in MIR155 HG overexpression group at 72 h（2.47±0.14）and 96 h（3.13±0.15）after transfection respectively,compared with vector control group at 72 h（2.09 ±0.12） and 96 h（2.50 ±0.13） after transfection（P =0.006,P =0.027;A value in MIR155 HG knockdown group at 48 h（1.69 ±0.15）,72 h（1.87 ±0.09） and 96 h（2.24 ±0.16） after transfection was lower than in negative control group at 48 h（2.04 ±0.06）,72 h（2.43 ±016） and 96 h（2.88 ±0.11） after transfection respectively（P =0.006,P =0.006,P =0.004）;The percentage of G0/G1 phase increased from 54.32% in negative control group to 63.93% in MIR155 HG knockdown group. Transwell migration assay indicated that the number of migrated cells in MIR155 HG overexpression group（31.67±3.51）was more than in vector control group（12.67±2.51）（P=0.002）;the number of migrated cells in MIR155 HG knockdown group（13.67 ±3.21） was less than in negative control group（36.00±4.58）（P=0.002）. Transwell invasion assay showed that the number of invaded cells in MIR155 HG overexpression group（42.33 ±7.02） was more than in vector control group（15.67 ±3.51）（P =0.004）;the number of invaded cells in MIR155 HG knockdown group（15.00 ±3.60） was less than in negative control group（39.00±4.36）（P=0.002）. Real-time PCR analysis showed that the fold change of mi R-155-5P expression in MIR155 HG overexpression group was upregulated to 17.99 ±2.42 compared with vector control group（P =0.000�
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2017年第1期21-26,共6页
Journal of Chongqing Medical University
基金
国家自然科学基金青年基金资助项目(编号:81502161)
重庆市科委基础科学与前沿技术研究(一般)资助项目(编号:cstc2015jcyj A10007)
