摘要
本研究以棉籽壳水不溶性粗木聚糖为底物,考察超声耦合酶解棉籽壳木聚糖制备低聚木糖的工艺条件。通过单因素实验以及Box-Behnken响应面实验对低聚木糖的制备工艺进行了优化。实验结果显示,最佳超声耦合酶解条件为时间42 min,温度45℃,反应体系p H=5.1,超声频率40 k Hz,超声强度0.22 W/cm2,还原糖总量达到最高50.46%。经HPLC分析,木二糖的含量占粗棉籽壳木聚糖的29.38%,占还原糖总量58.22%。
The study focused on ultrasound- coupled enzymatic hydrolysis to produce xylooligosaccharides from cottonseed husk by response surface methodology.The conditions of ultrasound- coupled enzymatic hydrolysis were optimized by response surface methodology,and its mathematical model was established. Results showed that the optimal parameters of ultrasound- coupled enzymatic hydrolysis were as follows： the initial p H5.1,hydrolysis time 42 min,temperature 45 ℃,ultrasonic frequency 40 k Hz,ultrasonic intensity 0.22 W / cm2. Under the optimal conditions,the conversion ratio of reducing sugar reached 50.46%.Moreover,the components of ultrasound- coupled enzymatic hydrolysis were measured by HPLC,the conversion ratio of xylobiose was 29.38%,accounted for 58.22% of the total reducing sugar.
出处
《食品工业科技》
CAS
CSCD
北大核心
2017年第4期186-190,共5页
Science and Technology of Food Industry
基金
国家自然科学基金项目(31201449)
国家自然科学基金项目(31371723)
关键词
棉籽壳
木聚糖
低聚木糖
响应面优化
cottonseed husk
xylan
xylooligosaccharides
response surface analysis
