摘要
Objective: To study the treatmaient of non-small cell lung cancer, we established the HU-Prim allograft transplantation tumor model. Methods: The fresh tumor samples were transplanted in the right scapular subcutaneous layer of the severe combined immunodeficient Non-obese diabetic/severe combined immunodeficient(NOD/SCID) mice. The pathological features of the tumors were observed. Nonnecrotic tissue was inoculated subcutaneously into the right axillary. When the tumor in burdened rat grew approximately 100 mm3, according to the tumor size all the animals were divided into the following four groups, eight rats in each group: solvent control group, gefitinib group(100 mg/kg), erlotinib group(50 mg/kg), afatinib group(20 mg/kg). Aniamals were treated with drugs by intragastric(i.g.) administrated, once daily, for consecutively 14 days. Measure the tumor size 2-3 times every week. Results: Hu Prime1-NSCLC mutant sensitive xenograft model research data showed that reversible tyrosine kinase inhibitors gefitinib, erlotinib and irreversible tyrosine kinase inhibitor afatinib could effectively inhibit tumor growth in EGFR positive NSCLC allografts model. The pharmacodynamic activity of irreversible inhibitor was better than that of the reversible inhibitor. Specimens from clinical anthropogenic tumor retain characteristics of the human primary malignancy, histopathology, biological characteristics, and tumor markers, etc., which can more accurately reflect the characteristics of the tumor and the impact of interventions. Conclusion: The model is not only a good antitumor drug experimental platform, but also a new evaluation tool of individualized medication.
Objective： To study the treatmaient of non-small cell lung cancer, we established the HU-Prim allograft transplantation tumor model. Methods： The fresh tumor samples were transplanted in the right scapular subcutaneous layer of the severe combined immunodeficient Non-obese diabetic/severe combined immunodeficient（NOD/SCID） mice. The pathological features of the tumors were observed. Nonnecrotic tissue was inoculated subcutaneously into the right axillary. When the tumor in burdened rat grew approximately 100 mm3, according to the tumor size all the animals were divided into the following four groups, eight rats in each group： solvent control group, gefitinib group（100 mg/kg）, erlotinib group（50 mg/kg）, afatinib group（20 mg/kg）. Aniamals were treated with drugs by intragastric（i.g.） administrated, once daily, for consecutively 14 days. Measure the tumor size 2-3 times every week. Results： Hu Prime1-NSCLC mutant sensitive xenograft model research data showed that reversible tyrosine kinase inhibitors gefitinib, erlotinib and irreversible tyrosine kinase inhibitor afatinib could effectively inhibit tumor growth in EGFR positive NSCLC allografts model. The pharmacodynamic activity of irreversible inhibitor was better than that of the reversible inhibitor. Specimens from clinical anthropogenic tumor retain characteristics of the human primary malignancy, histopathology, biological characteristics, and tumor markers, etc., which can more accurately reflect the characteristics of the tumor and the impact of interventions. Conclusion： The model is not only a good antitumor drug experimental platform, but also a new evaluation tool of individualized medication.
出处
《中国应用生理学杂志》
CAS
CSCD
2016年第6期525-528,共4页
Chinese Journal of Applied Physiology
基金
National Natural Science Foundation of Tianjin,China(16JCYBJC27500)
关键词
非小细胞肺癌
肿瘤模型
异种移植
药物治疗
酪氨酸激酶抑制剂
人类
原发性
组织病理学
non-small-cell lung cancer（NSCLC）
Non-obese diabetic/severe combined immunodeficient（NOD/SCID） mice
efficacy
tyrosine kinase inhibitors（TKIs）
disease model
