摘要
以榕属中黄金榕、橡皮树、小叶榕3个树种为研究对象,以其叶片为试材提取基因组DNA,进行ITS-PCR扩增。对扩增产物测序结果进行分析,在NCBI上选取同源率较高的27个树种的ITS序列与其进行同源比较,并建立系统树分析它们之间的亲缘进化关系。结果表明:30种榕属植物基于ITS序列分析与传统分类学略有差异,通过结合ITS鉴定与形态分类相结合可以为以后榕属的分类提供借鉴。
Using the leaves of Ficus microcarpa,F. elastic,F. concinna,F for ITS-PCR amplification,and then sequencing analysis was carried on. The ITS sequences（ 27 ITS sequences which had high scores with homology comparison were downloaded from NCBI） was used to build a phylogenetic tree to analyze their genetic evolutionary relationships. Resμlts showed that： It is different between based on ITS sequence analysis and traditional taxonomy,By combining the ITS identification and morphological classification can provide a reference for future classification of Ficus.
出处
《福建林业科技》
2016年第1期9-13,共5页
Journal of Fujian Forestry Science and Technology
基金
国家林业局948项目(2012-4-62)
国家自然科学基金项目(31360404)
